超滤法浓缩富集牡蛎蛋白抗氧化活性肽  

Preparation and enrichment antioxidant peptides from oyster protein hydrolysate using ultrafiltration

作　　者：郝更新[1,2,3] 曹文红[4] 郝记明[4] 章超桦[4] 

机构地区：[1]中国科学院南海海研究所,广东广州510301 [2]集美大学生物工程学院,福建厦门361021 [3]中国科学院研究生院,北京100049 [4]广东海洋大学食品科技学院,广东湛江524025

出　　处：《食品工业科技》2013年第11期77-80,共4页Science and Technology of Food Industry

基　　金：国家现代农业产业技术体系(CARS-48);集美大学李尚大基金(ZC2011016)

摘　　要：为研究牡蛎蛋白酶解产物中发挥抗氧化活性的肽片段,采用超滤的方法对其进行初步分离富集,测定了各级组分的体外抗氧化能力和氨基酸组成。结果表明:<4000u超滤液对羟自由基、DPPH自由基和超氧自由基清除活性的IC50值分别为17.29、2.82、7.02mg/mL,对肝脂质过氧化抑制作用的IC50值为14.40mg/mL,均低于原液和其他超滤组分;<4000u超滤液的还原力均高于同浓度的原液和其他超滤组分。经氨基酸分析,超滤后组氨酸、脯氨酸、甲硫氨酸、半胱氨酸、酪氨酸、色氨酸及苯丙氨酸的含量得到了富集。<4000u的超滤液经凝胶层析后,活性部分主要集中在550～3890u。In order to study the active fraction of oyster protein hydrolysate,it was preliminary separated and purified by using the ultrafiltration membrane and different antioxidant in vitro and amino acid assays were employed.It was found that the ultrafiltrate（〈4000u） acted as the scavenger for hydroxyl radical（ IC50 17.29mg/mL）, 1, 1-diphenyl- 2-picrylhydrazyl（ DPPH. ）（ IC50 2.82mg/mL）and superoxide anion radical（ IC50 7.02mg/mL）.And it also had the ability to inhibit lipid peroxidation in a linoleic acid emulsion system.The ultrafiltrate （ 〈 4000u）played a higher reducing power to equal concentration of hydrolysate and other ultrafiltrate fractions.Seven amino acids which may play an important role in antioxidant capacity, including Histidine, Proline, Methionine, Cysteine, Tyrosine, Tryptophan and Phenylalanine,were enriched by ultrafiltration.The main component with high antioxidant capacity was arranged in 550-3890u in the ultrafiltrate（〈4000u）.

关 键 词：牡蛎蛋白 酶解产物 超滤 抗氧化活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]