辣椒叶多酚纯化工艺研究  

Study on the purification craft of polyphenols from Capsicum annuum leaves

作　　者：郝鹏飞 黄昀[2] 李勇[2,3] 陈娜[1,2] 丁金祥[2,4] 辛敏[1,2] 唐劲天[2] 

机构地区：[1]北京中医药大学,北京100102 [2]清华大学工程物理系粒子技术与辐射成像教育部重点实验室,北京100084 [3]兰州交通大学化学与生物工程学院,甘肃兰州730070 [4]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品工业科技》2013年第11期206-209,共4页Science and Technology of Food Industry

基　　金：中国博士后科学基金(20100480315);国家自然科学基金青年基金项目(81102889)

摘　　要：目的:建立并优化辣椒叶多酚的纯化工艺。方法:利用静态吸附与解吸附实验筛选出最适宜纯化辣椒叶多酚的大孔树脂,并利用单因素方法考察最优柱色谱条件。结果:HPD-100型大孔树脂具有较高的吸附率和解吸率,并确定其最优色谱条件为:树脂柱径高比1:4,样品多酚浓度在0.6～1.2mg/mL之间,吸附速率2BV/h,以5BV/h的流速水洗3BV,再用3BV70%乙醇洗脱,合并洗脱液真空干燥即得。结论:该工艺对辣椒叶提取物中多酚的纯化简单高效,纯化后产物中多酚含量自4%提高到68%。Objective：To create and optimize the purification craft of polyphenols from Capsicum annuum leaves. Methods ：Macroporous resin was chosen under the condition of static adsorption and desorption,then using single -factor approach to study the optimal column chromatography conditions. Result： HPD-100 resin has better adsorption and desorption properties than that of other resins.The optimal column chromatography conditions. resin column diameter to height ratio 1：4,sample polyphenol concentration between 0.6 -.2g/mL, adsorption rate 2BV/h,adsorpted resin 5BV/h flow rate of water washing 3BV, and using 3BV of 70% ethanol for elution, then combine the elution, vacuum drying. Conclusion-This process was simple and efficient for purification the polyphenols from Capsicum annuum leaf extract. Following above conditions, the polyphenols purity increaseed from 4% to 68%.

关 键 词：辣椒叶 多酚 大孔吸附树脂 纯化工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]