苋籽ACE抑制肽的大孔树脂分离纯化  

Macroporous resin purification of Amaranth angiotensin-I converting enzyme(ACE)inhibitory peptides

作　　者：陈飞平[1,2] 周家华[1] 曾凡坤[2] 常虹[1] 

机构地区：[1]北京市农林科学院农业综合发展研究所,北京100097 [2]西南大学食品科学学院,重庆400715

出　　处：《食品工业科技》2013年第11期271-276,共6页Science and Technology of Food Industry

摘　　要：比较6种大孔树脂对苋籽ACE抑制肽的吸附-解吸效果,从中筛选出合适该活性肽分离纯化的树脂,并对其吸附-解吸工艺进行优化。结果表明,DA201-C树脂最适合苋籽ACE抑制肽的纯化,在样品质量浓度10mg/mL,pH为5,上样量1BV,流速6mL/min时,树脂的吸附效果最佳,吸附率达83.69%,再用5BV体积分数75%乙醇,以5mL/min的流速进行洗脱,此时几乎把吸附的多肽全部洗脱下来,解吸率为98.69%。经树脂纯化,样品的蛋白纯度为89.47%,脱盐率为88.86%,短肽含量提高了20.96%,ACE抑制活性提高了27.91%。The adsorption and desorption rates of 6 types of macroporous resins towards Amaranth angiotensin-I converting enzyme inhibitory peptides were investigated.Among the study, DA20!-C resin was the most suitable one to purify the bioactive peptides.When 1BV of 10mg/mL and pH5 sample was flowed at a speed of 6mL/min through DA2-1C resin,the adsorption effect was best with a adsorption rate reaching 83.69%.Then the adsorbed peptides could be almost exactly eluted with 5BV of 75% （V/V）ethanol at 5mL/min. After the purification, the protein content and salt rejection rate were 89.47% and 88.86% respectively,as well as the oligopeptides content and ACE inhibition were changed by 20.96% and 27.91%.

关 键 词：苋籽 ACE抑制肽 分离纯化 大孔树脂 

分 类 号：TS201.1[轻工技术与工程—食品科学]