高效液相色谱测定益智仁中的牛磺酸含量  

Determination of Taurine in Alpinia oxyphylla Miq. by High Performance Liquid Chromatography

作　　者：叶思平[1] 陈悦娇[1] 陈海光[1] 邓雅哲[1] 

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《食品科学》2013年第10期204-206,共3页Food Science

基　　金：广东省科技计划项目(2011B010500017)

摘　　要：建立高效液相色谱测定益智仁中牛磺酸含量的方法。经考察色谱流动相:甲醇-0.05mol/L的醋酸钠溶液(65:35,V/V),流速为1.0mL/min,DAD检测波长为UV-330nm。测定结果表明:样品中含有牛磺酸,线性范围为4～58mg/L,加标实验回收率为102.6%～105.3%,相对标准偏差为2.15%。益智仁中的牛磺酸的平均含量约为363.0mg/kg。A method to determine taurine in Alpinia oxyphylla Miq.by high performance liquid chromatography（HPLC） was described.The mobile phase for chromatographic separation was made up of methanol and 0.05 mol/L sodium acetate（65：35,V/V） at a flow rate of 1.0 mL/min.Detection wavelength was 330 nm.The results showed that dried fruits of Alpinia oxyphylla Miq.were detected to contain taurine by the HPLC method.This method demonstrated a linear range of 4 to 58 mg/L.Average recovery rates for taurine in spiked samples were between 102.6% and 105.3%,with RSD of 2.15%.The average taurine content in dried fruits of Alpinia oxyphylla Miq.was 363.0 mg/kg.

关 键 词：益智仁 高效液相色谱 牛磺酸 

分 类 号：TS272[农业科学—茶叶生产加工]