李斯特属细菌特征挥发性代谢物的鉴定分析  

Characterization of Volatile Metabolites of Listeria spp. Strains

作　　者：陈雪[1,2] 倪鹏[3] 喻勇新[1,2] 潘迎捷[1,2] 赵勇[1,2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]南通出入境检验检疫局,江苏南通226018

出　　处：《食品科学》2013年第10期231-237,共7页Food Science

基　　金：上海市科委部分地方院校能力建设项目(11310501100);江苏检验检疫局科技计划项目(2011KJ42);上海市科委工程中心建设项目(11DZ2280300)

摘　　要：采用气相色谱-质谱联用技术对李斯特属5种细菌的挥发性代谢产物进行研究,鉴定其化学物质,测定各物质的相对含量。研究发现通过与空白培养基比较,样品间共有挥发性物质有酮类(3种)、醛类(2种)、烷烃(2种)和其他物质(4种),且样品间各物质相对含量差异显著(P<0.05);经菌株代谢新产生挥发性物质有乙醇、丙酮、3-羟基-2-丁酮、乙酸丁酯、3-甲硫基丙醛和6-丁基壬烷;以及各菌株独有的挥发性代谢物质,最终发现每个细菌特有的气味化学条码。并通过电子鼻技术有效的区分李斯特属的5种细菌。研究表明,此气味化学条码有望应用于李斯特属细菌无损、快速的鉴定、检测。Here we analyzed for the firtst time volatile metabolites from five species of Listeria were systematically by gas chromatography-mass spectroscopy（GC-MS）.Cultures of all the Listeria species shared the common volatile metabolites： three ketones,two aldehydes,two alkanes and four other substances.There were significant differences in the relative content of the common volatile metabolites among these Listeria cultures（P 0.05）.The new volatile metabolites ethanol,acetone,3-hydroxy-2-butanone,butyl acetate,3-（methylthio） propanal and 6-butyl nonane as well as those unique to each strain were formed.Specific chemical barcodes were established for the unique smell of each strain.Effetive discrimination among the five Listeria strains was perfomed using electronic nose.The chemical barcodes hold promise for the nondestructive,rapid idfentification and detection of Listeria strains.

关 键 词：气相色谱-质谱联用 电子鼻 挥发性代谢产物 指纹图谱 

分 类 号：TS207.4[轻工技术与工程—食品科学]