Prevention of bone loss by injection of insulin-like growth factor-1 after sciatic neurectomy in rats  

作　　者：SUN Hai-biao CHEN Jun-chang 

机构地区：[1]Xi'an Jiaotong University Medical School, Xi'an 710049,China

出　　处：《Chinese Journal of Traumatology》2013年第3期158-162,共5页中华创伤杂志（英文版）

摘　　要：Objective： Injection of insulin-like growth factor-1 （1GF-1） can prevent bone loss in sciatic nerve transaction rats. We try to investigate the action mechanism of IGF-1 on bone formation. Methods： A total of 40 adult male Spragne-Dawley rats were divided into two groups （experimental group and con- trol group） with 20 animals in each. Sciatic neurectomy was performed to model disuse osteoporosis in all rats. IGF-1 was administered in experimental group with the dose of 100 gg/kg per day for 3 days. Meanwhile, the rats in control group were treated with saline. Bone mineral density was measured by dual-energy X-ray absorptiometry 4 and 6 weeks after neurectomy respectively. Expression of Osterix and Runx2 was determined by reverse transcription-poly- merase chain reaction （RT-PCR） assay. Results： There was a significant increase in the bone mineral density of experimental group compared with con- trol group. There was a significant decrease in the level of receptor activator of nuclear factor- K B-ligand but an increase in the level ofosteoprotegerin 4 and 6 weeks after neurectomy in the experimental group compared with con- trol one. The expression of Osterix and Runx2 was up-regu- lated in the bone marrow of experimental group compared with control group, Conclusion： IGF-1 can increase bone formation by stimulation of osteoblast number and activity, and reduce bone resorption by restriction of differentiation of osteoclast, suggesting that IGF-1 may improve the therapeutic efficacy for disuse osteoporosis.

关 键 词：OSTEOPOROSIS SCIATIC NERVE OSTEOBLASTS INSULIN-LIKE growth factor 1 

分 类 号：R641[医药卫生—外科学]