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机构地区:[1]武汉生物工程学院,湖北武汉430415 [2]长江大学生命科学学院,湖北荆州434025
出 处:《安徽农业科学》2013年第10期4626-4628,共3页Journal of Anhui Agricultural Sciences
基 金:武汉市教育局项目(2006K011)
摘 要:[目的]制备大米多孔淀粉,测定其吸附性能。[方法]以浸碱法自制的大米淀粉为原料,采用糖化酶、α-淀粉酶复合酶水解的方法制备大米多孔淀粉,以吸油率、比孔容及对桔子香精的缓释性能等指标评价大米多孔淀粉吸附性能。[结果]制备大米多孔淀粉的最佳酶解工艺条件为:反应温度35℃,时间16 h,pH 4.5,糖化酶、α-淀粉酶酶配比10∶1,底物浓度为0.2 g/ml,颗粒粒度40目。在此条件下制备的大米多孔淀粉吸油率最高,达到58.14%。[结论]大米多孔淀粉有较高的吸油率,较大的比孔容,较好的缓释桔子香精的功能,可作为多种物质的吸附载体并广泛应用。研究可为我国大米资源综合开发提供有效途径,并对我国的多孔淀粉工业化生产起到一定推动作用。[Objective] To prepare porous starch of rice and measure its adsorption performance.[Method] With immersion home-made rice starch as raw material,adopting saccharifying enzyme,compound of alpha amylase enzyme hydrolysis to prepare porous starch.With oil absorption rate,specific pore volume and the slow release of orange essence performance as indicators to evaluate rice porous starch.[Result]The best digestion process conditions were as follows: reaction temperature 35 ℃,time 16 h,pH 4.5,ratio of saccharifying enzymealpha and amylase enzyme 10∶ 1,the substrate concentration 0.2 g/ml,grain size 40 mesh.Under this condition,the preparation of porous starch of rice oil absorption rate is the highest,up to 58.14%.[Conclusion] Porous starch of rice have higher oil absorption rate,larger specific pore volume and good slow release of orange essence,which can be used extensively as adoption carrier for many substances,providing an effective way for comprehensive utilization of rice resource and promoting industrial production of porous starch.
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