儿茶素对羟自由基诱发 SOD 基因序列改变的影响  

Effects of catechins on spectrum changes of SOD gene induced by hydroxyl radical

作　　者：沈生荣[1] 赵玉芳[2] 凌备备 陈子元[2] 

机构地区：[1]浙江大学茶学系,浙江杭州310029 [2]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2000年第1期25-29,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金资助项目

摘　　要：利用基因克隆与测序技术 ,研究了儿茶素对羟自由基诱发 SOD基因序列改变的影响 ,结果表明 :Fe( ) /H2 O2 产生的羟自由基可诱导西红柿叶片 SOD基因中 G→ A,G→ C间的颠换 ,且 Fe( )浓度越高 ,颠换率越高 ,尤其是 G→ C的颠换 ,但儿茶素的加入明显抑制这种颠换 ,且 (一 ) - EGCG>(一 ) -ECG>(一 ) - EGC>(一 ) - EC,呈浓度—效应关系 ,而儿茶素本身不能使By employing the technique of gene cloning and DNA seqencing the effects of hydroxyl radical produced by Fe (Ⅱ) / H 2O 2 reaction were exploring on the spectrum of SOD gene from tomoto leaf and influenced on by catechins. The results showed that the spectrum of Fe (Ⅱ) / H 2O 2 induced changes detected in SOD gene clong assay. The most frequent single base substitution was G→C and G→A. But the ratio of this transition was increased with increasing the concentration of Fe (Ⅱ). It has seriously influence on G→C transition. Moreover, the changes were prohibited after added catechins to the system. The order of prohibition could be classified as follows: (一) EGCG>(一) ECG>(一) EGC>(一) EC. The transitions induced by Fe (Ⅱ) / H 2O 2 were selectively decreased in the presence of catechins and existed the relations between concentration of catechins and effects. Alternatively, catechins by itself could not transit the single base sequence of SOD gene spectrum.

关 键 词：儿茶素 羟自由基 DNA碱基颠换 SOD基因 基因突变 

分 类 号：Q754[生物学—分子生物学] S571.101[农业科学—茶叶生产加工]