鲍鱼生殖腺多糖脱硫酸化及光谱鉴定  

Desulfation and optical spectral verification of a polysaccharide from abalone gonad

作　　者：宋叶涵[1] 刘富俊[2] 李冬梅[1] 杨静峰[1] 朱蓓薇[1] 

机构地区：[1]大连工业大学国家贝类加工技术研究分中心(大连),辽宁大连116034 [2]辽宁省大连海洋渔业集团公司,辽宁大连116113

出　　处：《大连工业大学学报》2013年第1期1-3,共3页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(31171635)

摘　　要：以鲍鱼生殖腺多糖为原料,通过对稀酸水解法、一步脱硫法和二步脱硫法进行比较,确定了鲍鱼生殖腺多糖硫酸基脱除的最适方法。经过二步脱硫法制备得到的鲍鱼生殖腺脱硫多糖,在红外光谱的1 253cm-1处(SO24-特征吸收峰)基本消失,硫酸取代基脱除率为87.64%,多糖回收率为50%,并且多糖分子的母体结构未被破坏。二步脱硫法为鲍鱼生殖腺多糖脱除硫酸基的最适方法。The desulphated comparison of abalone gonad polysaccharide by diluted acid hydrolysis, one-step desulfurization or two-step desulfurization showed that two-step desulfurization gave the best sulfate removal abilities. The desulfated polysaccharide caused the elimination of the huge infrared SO42-absorption peaks （1 253 cm-1）, and removal ratio is 87.64%, indicating that the parent structure was not changed. Two-step desulfurization is considered as proper method for the sulfate removal of the abalone gonad polysaccharide.

关 键 词：鲍鱼生殖腺多糖 硫酸基 脱硫酸 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]