纤维废渣酶水解及L-乳酸发酵的研究  

Study on Enzymatic Hydrolysis Using Lignocellulosic Residue as Raw Materials for L-Lactic Acid Fermentation

作　　者：陈育如[1] 夏黎明[2] 岑沛霖[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]浙江大学生物化工研究所,浙江杭州310027

出　　处：《高校化学工程学报》2000年第5期437-441,共5页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金资助项目!(编号29876036)。

摘　　要：木糖厂的纤维废渣中,纤维素为主要成份, 本文用纤维素酶对木糖厂的纤维废渣进行酶解，继而研究了用米根霉进行乳酸发酵。结果表明，实验条件下，纤维废渣可用纤维素酶直接水解或经碱处理后进行酶水解, 9%的纤维废渣经酶解后还原糖浓度为39.6mg·mL-1，纤维素糖化率为68.2%. 物料经碱处理后酶解液的还原糖浓度为48.2mg·mL-1, 比碱处理前提高了约16.1%。水解液用米根霉进行乳酸发酵，乳酸的浓度达到了29.5mg·mL-1，比用碱处理前物料酶解液乳酸发酵的乳酸浓度(24.0mg·mL-1)增加了约23%，但过程的总体效率有待进一步提高。Cellulose is the main component in lignocellulosic residue which comes from xylose production industry. The enzymatic hydrolysis by cellulase and lactic acid fermentation of lignocellulosic residue by Rhizopus oryzae were studied experimentally. With the 9% of initial lignocellulosic residue concentration, the reduce sugar concentrations after enzymatic hydrolysis with or without alkali pretreatment were 39.6 and 48.7mg·mL-1 respectively, and 24.0mg·mL-1 and 29.5mg·mL-1 of lactic acid could be further obtained by fermentation with Rhizopus oryzae. The lactic acid yields were 57.8% and 61.2% based on total sugar in hydrolysate respectively. The experimental results indicated that the alkali pretreatment of lignocellulosic residue from xylose production industry was favorable for both enzymatic hydrolysis and L-lactic acid fermentation.

关 键 词：纤维废渣 纤维素酶 酶水解 米根霉 乳酸发酵 

分 类 号：X783.505[环境科学与工程—环境工程] TQ921.3[轻工技术与工程—发酵工程]