条斑紫菜与坛紫菜蛋白组成与生物活性比较研究  

Protein Composition and Biological Activity of Lavers Porphyra yezoensis and P. haitanensis

作　　者：姚兴存[1] 王洪斌[1] 舒留泉[1] 岳清清 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005

出　　处：《水产科学》2013年第4期215-218,共4页Fisheries Science

基　　金：中央财政支持地方高校发展专项资金项目(CXTD12)

摘　　要：以条斑紫菜和坛紫菜为原料,采用清除羟自由基、二苯代苦味酰基自由基和总还原力为生物活性评价指标,比较2个种类紫菜的蛋白抗氧化活性,并利用聚丙烯酰胺凝胶电泳分析法比较蛋白组成的差异。结果表明,2个种类的紫菜蛋白均具有良好的生物活性,条斑紫菜蛋白的清除羟自由基能力强于坛紫菜;条斑紫菜和坛紫菜蛋白在清除二苯代苦味酰基自由基能力方面未显示出明显差异,但同种类的不同产地之间差异显著;紫菜蛋白清除二苯代苦味酰基自由基的能力大于清除羟自由基能力;条斑紫菜蛋白的总还原能力强于坛紫菜蛋白。聚丙烯酰胺凝胶电泳结果显示,不同产地条斑紫菜之间蛋白质组成差异较大,而坛紫菜不同产地之间蛋白质组成相同,条斑紫菜和坛紫菜在蛋白质组成及分子质量方面具有明显差别。Abstract： The protein composition was analyzed in lavers Porphyra yezoensis and P. haitanensis by SDS- PAGE, and the antioxidative activity including against hydroxyl radicals, and DPPH radicals and reducing activities of different kinds of laver proteins were studied in the layers. The results showed that two laver proteins had a good biological activity, and there was higher hydroxyl radicals scavenging capacity in P. yezoensis than that in P. haitanensis. No significant difference in the DPPH scavenging activity was observed in the laver proteins, with significant differences in the different varieties. P. yezoensis showed greater ability to scavenge DPPH and the reducing capacity than ability to scavenge hydroxyl radicals, than Porphyra haitanensis did. SDS-PAGE showed that different P. yezoensis proteins were different in composition, and P. haitanensis protein composition was the same in different regions. P. yezoensis and P. haitanensis were found to be different in protein composition.

关 键 词：条斑紫菜 坛紫菜 蛋白组成 生物活性 

分 类 号：S986.2[农业科学—捕捞与储运]