单核细胞增生李斯特氏菌拮抗菌的分离鉴定及其抑菌活性  

Isolation and Identification of Antagonistic Strain against Listeria monocytogenes

作　　者：许红岩 段效辉 李小清[2] 

机构地区：[1]烟台出入境检验检疫局,山东烟台264000 [2]临沂市技术监督信息所,山东临沂276000

出　　处：《食品科学》2013年第15期181-185,共5页Food Science

基　　金：国家质检总局科研项目(2005IK022)

摘　　要：从烟台近郊土壤中分离出一株单核细胞增生李斯特氏菌拮抗微生物sly-3,利用传统生理生化和分子生物学相结合的方法对其进行鉴定,并采用生物测定的方法评价其抑菌活性。菌株的形态学特征和生理生化特征与枯草芽孢杆菌基本一致,其16S rDNA序列与GenBank中已鉴定的枯草芽孢杆菌的16S rDNA序列同源性达99%以上,据此初步确定其为枯草芽孢杆菌。抑菌谱研究发现,该菌仅对革兰氏阳性菌有抑制作用,而对革兰氏阴性菌无明显抑制作用。sly-3菌株发酵过滤液初步作用于水产品中单核细胞增生李斯特氏菌,发现可有效抑制该致病菌的生长,且在高温处理条件下保留较高的抑菌活性,在食品保鲜中显示出了良好的应用前景。In this study, sly-3 strain that can inhibit Listeria monocytogenes was isolated from soil in Yantai suburb. The morphological, physiological and biochemical characteristics of the isolated strain were basically consistent with those of Bacillus subtilis and the homology of 16S rDNA sequence between sly-3 and B. subtilis CICC10034 was 99%. On the basis of these results, sly-3 was finally identified as Bacillus subtilis. The antagonistic activity of sly-3 strain also suggested that only G＋ bacteria could be inhibited but G- bacteria could not be inhibited. Meanwhile, the 24 h culture filtrate of sly-3 exhibited high inhibitory activity against Listeria monocytogenes from export aquatic products.

关 键 词：水产品 单核细胞增生李斯特氏菌 拮抗菌 

分 类 号：Q939.92[生物学—微生物学]