检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:曾文亮[1] 杨金易[1] 王弘[1] 沈玉栋[1] 罗林[1] 曾道平[1] 徐振林[1] 孙远明[1]
机构地区:[1]广东省食品质量安全重点实验室,农业部农产品贮藏保鲜质量安全风险评估实验室,华南农业大学食品学院,广东广州510642
出 处:《食品工业科技》2013年第16期70-74,共5页Science and Technology of Food Industry
基 金:农业部公益性行业专项(201003008-08);广东省教育部产学研项目(2011A090200029);福建省科技计划重点项目(2012Y0003)
摘 要:应用胶体金免疫层析技术,制备苯甲酸胶体金免疫层析快速检测试纸条,采用柠檬酸三钠还原法制备颗粒直径为17nm的胶体金,将苯甲酸金标抗体喷涂在玻璃纤维垫上制成胶体金结合垫,将包被抗原和二抗羊抗兔分别结合于硝酸纤维膜作为检测线(T线)和质控线(C线),组装成胶体金免疫层析试纸条并进行灵敏度测试。结果显示,胶体金试纸条检测限为100μg/L,可在5~7min内完成测试,与苯酚、甲苯、苯乙酸、苯丙氨酸等药物均无交叉反应,检测结果与酶联免疫吸附法完全一致。该方法具有操作简便、快速、特异性强、灵敏度高等特点,适用于现场快速检测和筛选工作,具有广泛的商业开发应用前景。Colloidal gold immune chromatography technology was applied in this study,the colloidal gold immunochromatographic test strip was developed in order to establish a rapid detection assay of benzoic acid. The colloidal gold with particle diameter 17nm was obtained by reducing gold chloride with sodium citrate, benzoic acid gold-labeled antibody was spotted on some chosen glass fiber made of colloidal gold combined pad,and the antigen of benzoic acid and Goat anti Rabbit IgG were coated on the nitrocellulose membrane to be the test line(T line) and the control line(C line). Test strips were constructed and the detection sensitivity was measured. Results showed that the detection limit of colloidal goldimmunochromatographic test strip was 100μg/L and the whole analysis process could be completed within 5 ~7min. With phenol,toluene,benzene acetic acid,phenylalanine and other drugs had no cross reaction:Results were in complete accord with enzyme-linked immunosorbent method. The method had simple operation,fast characteristics,strong specificity, high sensitivity. The colloidal gold immunochromatographic test strip could be widely used for On -site rapid detection and screening work and had great prospectfor commercial development.
分 类 号:TS207.3[轻工技术与工程—食品科学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:3.23.101.186