进口乳制品中克罗诺阪崎肠杆菌分离株耐药性研究  

Study on antibiotic resistance of Cronobacter sakazakii isolated from imported dairy product

作　　者：张西萌[1] 曾静[1] 魏海燕[1] 付溥博[1] 韩笑[1] 

机构地区：[1]北京出入境检验检疫局,北京100026

出　　处：《中国食品卫生杂志》2013年第4期320-323,共4页Chinese Journal of Food Hygiene

基　　金：国家质量监督检验检疫总局科研项目(2010IK190)

摘　　要：目的对进口乳制品中克罗诺阪崎肠杆菌分离株的耐药性进行研究。方法采用纸片扩散法对99株克罗诺阪崎肠杆菌分离株和1株标准菌株进行药敏性试验,共选择7大类20种抗生素。结果所测试的100株菌株对美洛西林、亚胺培南、美罗培南、庆大霉素、阿米卡星、卡那霉素、妥布霉素、氯霉素、头孢吡肟、头孢哌酮、头孢噻肟、头孢他啶、环丙沙星和诺氟沙星敏感;对苯唑西林产生耐药;对头孢噻吩、氨苄西林、头孢唑啉、和四环素具有不同程度的耐药性,耐药率分别为65.0%、17.0%、3.0%和2.0%;对头孢唑啉、头孢噻吩、氨苄西林、头孢曲松和四环素中介率分别为25.0%、23.0%、6.0%、2.0%和1.0%;13株对3种抗生素耐药,4株表现多重耐药性。结论进口乳制品中克罗诺阪崎肠杆菌分离株对所测试的大多数抗生素敏感,但对苯唑西林全部耐药,对部分抗生素出现较高的耐药和多重耐药性,因此克罗诺阪崎肠杆菌的耐药性应引起广泛的社会关注。Objective To investigate antibiotic resistance of Cronobacter sakazakii isolated from imported dairy products.Methods 100 strains of Cronobacter sakazakii were tested for antibiotic susceptibility by disk diffusion recommended by the National Committee of Clinical laboratory Standard.Results All strains were sensitive to Mezlocillin,Imipenem,Meropenem,Gentamicin,Amikacin,Kanamycin,Tobramycin,Chloramphenicol,Cefepime,Cefoperazone,Cefotaxime Sodium,Ceftazidime,Pentahydrate,Ciprofloxacin and Norfloxacin,while resistant to Proctaphlin Sodium.The ratio of resistant strains to Ampicillin,Cefalotin,Cefazolin Sodium,Sodium and Tetracycline was 65%,17%,3% and 2%,respectively.The ratio of intermediate resistant strains to Ampicillin,Cefazolin Sodium,Cefalotin Sodium,Ceftriaxone Sodium,Tetracycline was 25%,23%,6%,2% and 1% respectively.13 strains were multiresistant to 3 kinds of antibiotics and 4 strain was multiresistant.Conclusion Cronobacter sakazakii strains isolated from imported dairy products were susceptible to most of the tested antibiotics,but resistance were increasing.In this study,All drug resistant strains to oxacillin.Hereby,the issue of Cronobacter sakazakii multiresistance should arouse abroad attention worldwide.

关 键 词：乳制品 克罗诺阪崎肠杆菌 耐药性 食源性致病菌 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS201.3[医药卫生—公共卫生与预防医学]