不同pH值酿酒酵母分批发酵生产谷胱甘肽数学模型建立  

Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH

作　　者：郑丽雪[1,2] 谢群凡 王立梅[1,2] 齐斌[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]苏州市食品生物技术重点实验室,江苏常熟215500

出　　处：《食品科学》2013年第13期162-164,共3页Food Science

基　　金：国家自然科学基金面上项目(31171758);2011年度常熟理工学院"青蓝工程"优秀青年骨干教师培养对象项目

摘　　要：以麦芽汁为发酵培养基,考察不同pH值条件下酿酒酵母CS10515-1分批发酵合成谷胱甘肽的影响,对实验数据进行分析并拟合出数学模型。结果表明:两种pH值条件下高斯拟合对谷胱甘肽产量数据的拟合效果较好,相关系数(R2)分别为0.99235(pH5.0)、0.98842(pH6.0),而对生物量、基质消耗拟合效果差。另外,当发酵pH值恒定在5.0时,32h时谷胱甘肽产量达最大,为72mg/L,此时生物量为4.59g/L;当发酵pH值恒定为6.0时,同样也是32h时谷胱甘肽产量达最大,为91mg/L,此时生物量为4.11g/L。A mathematical model of glutathione production by Saccharomyces cerevisiae Y518 in batch fermentation at different pH was established with malt sprout extract as the fermentation medium.The results showed that Gaussian fitting model was in good agreement with the experimental observations for glutathione production at different pH,with correlation coefficient（R2） of 0.99235（pH5.0） and 0.98842（pH6.0）,but not suitable for biomass and substrate consumption prediction.Glutathione production reached the maximum of 72 mg/L at 32 h yielding a biomass of 4.59 g/L when pH was kept constant at 5.0.In contrast,maximum glutathione production of 91 mg/L was also obtained at 32 h along with a biomass of 4.11 g/L when pH was kept constant at 6.0.

关 键 词：谷胱甘肽 麦芽汁 酿酒酵母 高斯拟合 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]