正交试验优化向日葵盘果胶的提取和基本分析  

Optimization by Orthogonal Array Design of Sunflower Head Pectin Extraction and Characterization

作　　者：王琨[1] 华霄[2] 杨瑞金[1,2] 康佳琪[1] 张文斌[2] 赵伟[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学糖化学与生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品科学》2013年第14期1-5,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD23B03);江南大学自主科研项目

摘　　要：研究向日葵盘果胶(SFHP)的提取工艺。通过单因素试验,比较酸化水、0.75%六偏磷酸钠、0.5%乙二胺四乙酸二钠、0.5%草酸-草酸铵、0.5%草酸和0.5%草酸铵6种提取剂的提取效果,确定较优的提取剂种类。选择pH值、提取温度、提取时间和提取固液比为主要工艺参数,通过正交试验,确定较优的提取参数组合:pH3.50、提取时间45min、提取温度85℃、固液比1:30(g/mL)。在此条件下,向日葵盘果胶得率为20.9%(以半乳糖醛酸计)。采用高效尺寸排阻色谱-多角度激光散射检测器(HPSEC-MALLS)和高效阴离子交换色谱-脉冲安培检测器(HPAEC-PAD)测定了向日葵盘果胶的分子质量和单糖组成。结果表明,向日葵盘果胶的分子质量高于柑橘果胶,半乳糖醛酸为最主要单糖成分,占单糖总量的82.1%,鼠李糖为次要成分,仅占单糖总量的9.1%。The extraction of pectin from sunflower head was investigated.Different solvents were employed to extract sunflower head pectin（SFHP）.Major experimental conditions such as pH,temperature,extraction time,and solid-liquid ratio were explored.Based on single-factor tests,the extraction parameters were further optimized using orthogonal tests.Under the optimal extraction conditions： pH 3.5,45 min,85 ℃ and a solid-liquid ratio of 1：30（g/mL）,the yield of SFHP in the form of galacturonic acid were up to 20.9%.High performance size exclusion chromatography-multi-angle laser light scattering（HPSEC-MALLS） and high performance anion exchange chromatography-pulsed amperometric detection（HPAEC-PAD） were used to determine the molecular weight and monosaccharide compositions of SFHP.The molecular weight of SFHP was higher than that of citrus fruits and galacturonic acid was the major monosaccharide component of SFHP,amounting for 82.1% of the total monosaccharides.Rhamnose was the second major monosaccharide,accounting for only 9.1% of the total monosaccharides.

关 键 词：向日葵盘 低甲氧基果胶 分子质量 单糖组成 

分 类 号：TS202.1[轻工技术与工程—食品科学]