霸王花水溶性多糖提取工艺及其对羟自由基的清除作用  

Optimal Extraction and Hydroxyl Radical Scavenging Activity of Water-Soluble Polysaccharides from Pitaya Flowers (Hylocereus undatus (Haw.) Urilt. et. Rose)

作　　者：胡位荣[1] 孙茹[1] 李昭露[1] 江月玲[1] 刘顺枝[1] 

机构地区：[1]广州大学生命科学学院,广东广州510006

出　　处：《食品科学》2013年第14期104-107,共4页Food Science

基　　金：广东省科技计划项目(2010B020305014);广东省高等院校学科建设专项(2008-342);广州市属高校科研计划项目(2012A047)

摘　　要：以浸提时间、固液比、浸提温度、提取次数为影响因素,多糖得率为考察指标,在单因素试验的基础上进行正交试验,优化霸王花干制品水提醇沉法提取多糖的工艺条件,并探讨多糖清除羟自由基(.OH)活性。结果表明:霸王花多糖浸提最佳工艺为样品先用乙醇回流处理1h,按固液比1:100(g/mL)加入蒸馏水,90℃水浴浸提4h,浸提1次;浸提液经过醇析后,多糖得率9.59%。对所得多糖的溶解性、吸收光谱等理化性质进行初步观测,霸王花多糖质量浓度为6.4mg/mL时对Fenton体系产生的.OH清除率达50%,15.1mg/mL时可清除90%的.OH。Based on single-factor tests,optimal extraction conditions of hot water extraction and ethanol precipitation of polysaccharides from dried pitaya flowers（Hylocereus undatus（Haw.） Urilt.et.Rose） were explored by orthogonal array design.Factors including extraction duration,temperature,solid-solvent ratio and extraction times were selected to maximize the extraction rate of polysaccharides.The salicylic acid method was used to inspect the hydroxyl radical-scavenging activity of polysaccharides from pitaya flowers.The results showed that the optimal parameters for extracting polysaccharides from pitaya flowers were one cycle of extraction at 90 ℃ for 4 h with distilled water at a solid-to-solvent ratio of 1：100（g/mL） and after ethanol reflux pretreatment for 1 h.Under the optimal extraction conditions,the extraction rate of polysaccharides after ethanol precipitation was 9.59 %.The resulting polysaccharide extract was evaluated for solubility and spectral properties and it was found that its hydroxyl radical-scavenging rate was 50% at 6.4 mg/mL.

关 键 词：霸王花 水溶性多糖 浸提 羟自由基 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]