响应面法同步优化海带中褐藻糖胶和褐藻酸提取工艺  

Optimization of Extraction Process for Fucoidan and Alginc Acid from Laminaria japonica by Response Surface Methodology

作　　者：王明艳[1] 陈丽[1] 张诗山 沈春阳[1] 喻川[1] 

机构地区：[1]淮海工学院江苏省海洋资源开发研究院,江苏连云港222005 [2]天天海藻工业有限公司,江苏连云港222110

出　　处：《食品科学》2013年第14期135-140,共6页Food Science

基　　金：连云港市科技攻关项目农业攻关课题项目(CG1133);江苏省海洋生物技术重点建设实验室开放基金项目(2010HS11);淮海工学院大学生创新项目

摘　　要：在单因素试验基础上,以海带中褐藻糖胶和褐藻酸得率(Y1和Y2)为响应值,利用响应面法对海带中褐藻糖胶和褐藻酸提取工艺进行优化。采用响应面法绘制三维及等高线叠加图,应用同步优化程序,得出海带中褐藻糖胶和褐藻酸得率均高的最佳提取工艺参数为:浸提温度77℃、浸提时间4.4h、液料比52.0:1、消化温度48℃、消化液质量浓度2.2g/100mL、消化时间4.3h。在此工艺条件下,褐藻糖胶得率为2.58%,褐藻酸得率为28.11%,与数学模型计算所得预测值接近。可见,响应面同步优化法对海带中褐藻糖胶和褐藻酸提取条件进行同时优化合理可行。Based on the results of single factor tests,the extraction conditions of fucoidan and alginc acid from Laminaria japonica were optimized by response surface methodology（RSM） using the extraction rates of fucoidan and alginc acid（Y1 and Y2） as response values.Based on three dimensions and overlay contour plots,the optimal extraction conditions included extraction temperature of 77 ℃,extraction time of 4.4 h,liquid-solid ratio of 52.0：1,digestion temperature of 48 ℃,alkali liquor concentration of 2.2 g/100 mL,and digestion time of 4.3 h.Under the optimal conditions,the extraction rates of fucoidan and alginc acid were 2.58% and 28.11%,respectively,which were in close agreement with the predicted values.The RSM is practicable for optimizing extraction process.This method will improve the extraction conditions of fucoidan and alginc acid from Laminaria japonica.

关 键 词：海带 褐藻糖胶 褐藻酸 提取 响应面法 叠加等高线图 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]