利用HPLC-MS/MS测定三叶委陵菜中的黄酮类化合物  

Determination of Flavonoids from Potentilla freyninan by HPLC-MS/MS

作　　者：李荣[1] 周媛[1] 杨立琛[1] 姜子涛[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室,天津300134

出　　处：《食品科学》2013年第14期263-266,共4页Food Science

基　　金：天津市高校科技发展基金计划项目(20110608);天津市自然科学基金重点项目(120029)

摘　　要：利用75%乙醇作为溶剂,采用微波辅助法提取三叶委陵菜黄酮,黄酮样品经D101树脂纯化,采用高效液相色谱分离、串联四极杆质谱负离子方式检测分析。根据相对分子质量、二级质谱碎片、液相色谱保留时间等信息,从三叶委陵菜中测定出了二苯乙烯苷、槲皮素-3,7-芸香糖鼠李糖苷、牡荆素、芦丁、金丝桃苷、橙皮苷、槲皮苷和山奈酚-3-芸香糖苷8种黄酮类成分。Total flavonoids were obtained from Potentilla freyninan by microwave-assisted extraction method using 75% ethanol as the extraction solvent and purified with D101 macroporous resin.The purified total flavonoids were analyzed by high performance liquid chromatography tandem quadrupole mass spectrometry（HPLC-MS/MS） in the negative ion monitoring mode.Based on relative molecular weight,MS2fragmentation pattern,and retention time in liquid chromatography,eight flavonoids in P.freyninan were identified,including 2,3,5,4’-tetrahydroxystilbane-2-O-β-D-glucoside,quercetin-3,7rutinosogalactoside,apigenin-8-C-glucoside,rutin,hyperoside,hesperidin,quercitrin,and kaempferol-3-rutinoside.

关 键 词：三叶委陵菜 液相色谱-质谱 质谱联用 黄酮 橙皮苷 金丝桃苷 

分 类 号：TS207.3[轻工技术与工程—食品科学]