脂肪酶催化竹叶抗氧化物中异荭草苷和异牡荆苷酰化  

CandidaantarcticaLipase B-Catalyzed Acylation of Isoorientin and Isovitexin in Antioxidant of Bamboo Leaves (AOB)

作　　者：马祥[1] 晏日安[1] 逯与运[1] 

机构地区：[1]暨南大学食品科学与工程系,广东广州510632

出　　处：《食品科学》2013年第16期265-269,共5页Food Science

基　　金：广东市科技计划项目(11C12070753);广东省科技计划项目(2010A080403005)

摘　　要：研究南极假丝酵母脂肪酶在叔戊醇中催化竹叶抗氧化物中异荭草苷和异牡荆苷的酰化。反应条件为脂肪酶用量50g/L、分子筛4用量100g/L、竹叶抗氧化物中黄酮苷与月桂酸的物质的量比1:4、反应温度60℃、搅拌转速150r/min、反应时间48h。经过高效液相色谱分析,制备液相分离,得到2种被酰化的黄酮苷。对产物的结构采用电喷雾电离质谱分析,1H-NMR及13C-NMR表征,确定产物分别为异荭草苷-6″-月桂酸酯和异牡荆苷-6″-月桂酸酯。The acylation catalyzed by Candida antarctica lipase B of isoorientin and isovitexin in antioxidant of bamboo leaves（AOB） in tert-amyl alcohol as reaction medium was studied.The reaction condition was as follows： 50 g/L lipase,100 g/L molecular sieve 4,a molar ratio of flavonoids in AOB to laurate acid of 1：4,60 ℃,150 r/min stirring and a reaction duration of 48 h.After being analyzed and separated by high-performance liquid chromatography,two types of acylated flavonoids were obtained.Confirmation of chemical structures was performed by ESI-MS,1H-NMR and 13C-NMR and the two types of acylated flavonoids were isoorientin-6″-laurate and isovitexin-6″-laurate.

关 键 词：脂肪酶 异荭草苷 异牡荆苷 高效液相色谱 酰化 

分 类 号：TS202.3[轻工技术与工程—食品科学]