猪肌肉组织MDH和LPL基因表达与肌内脂肪含量和脂肪酸组成关系的研究  

The Gene Expression of MDH and LPL in Muscle and Their Association with Content of Intramuscular Fat and Composition of Fatty Acids in Pigs

作　　者：祝仁铸[1] 尹逊河[1] 王元虎 李少青 武道留[3] 王倩[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东绿涧生态产业园,章丘250200 [3]山东省农业管理干部学院,济南250100

出　　处：《畜牧兽医学报》2013年第8期1182-1188,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省科技攻关项目(2010GNC10930)

摘　　要：为了研究野莱F1代猪肌内苹果酸脱氢酶(Malate dehydrogenase,MDH)和脂蛋白脂酶(Lipoprotein li-pase,LPL)基因表达与肌内脂肪含量及脂肪酸组成的关系,探讨肌内脂肪的沉积机理。本研究以长白山野猪与莱芜猪的杂交1代猪(简称野莱F1代猪)和长×大白猪(各40头,公母各半)为研究对象,在20、35、50、70、90kg 5个体重阶段采取背腰最长肌,利用荧光定量RT-PCR方法研究MDH和LPL基因表达的发育性变化,分析该变化与肌内脂肪含量和脂肪酸组成的关系。结果表明:随着体重的增加,野莱F1猪和长×大白猪肌内MDH基因表达在20～70kg阶段呈上升趋势,在70～90kg阶段呈下降趋势;野莱F1猪和长×大白猪肌内LPL基因表达皆呈持续上升的趋势。相关分析表明:野莱F1猪MDH和LPL mRNA表达均与肌内脂肪含量呈显著正相关(P<0.05),与多不饱和脂肪酸/饱和脂肪酸的比值(PUFA/SFA)不相关(P>0.05);长×大白猪MDH和LPL mRNA表达分别与肌内脂肪含量不相关和显著正相关(P>0.05和P<0.05),分别与PUFA/SFA呈极显著和显著负相关(P<0.01和P<0.05);2种猪肌内PUFA/SFA与肌内脂肪含量皆呈显著负相关(P<0.05)。结果提示:猪肌内MDH和LPL基因表达皆具有明显的体重发育特征;可以通过控制猪饲料中PUFA的含量来调节猪肉中PUFA的含量,从而影响MDH和LPL的基因表达,达到调节肌内脂肪含量的目的。This experiment was conducted to study the relationship among gene expression of malate dehydrogenase and lipoprotein lipase in muscle and contents of intramuscular fat and composition of fatty acids in F1 hybrids with boar × Laiwu pigs,and to investigate the sedimentary mechanism of intramuscular fat.F1 hybrids with boar × Laiwu pigs（called YL F1） and pigs with Landrace × Yorkshire（40 pigs for each,20 for male,20 for female） were used.The dorsal-waist longissimus muscle were collected when the body weight reached 20,35,50,70 and 90 kg.The developmental changes of MDH and LPL genes expression was detected by fluorescent quantitative RT-PCR method,and the relationship between the change and the content of intramuscular fat and composition of fatty acids was analyzed.The results indicated that with the increase of body weight,the MDH gene expression in muscle in F1 hybrids with boar × Laiwu pigs and pigs with Landrace × Yorkshire pigs increased from 20 to 70 kg,and decreased from 70 to 90 kg.The LPL gene expression in muscle in F1 hybrids with boar × Laiwu pigs and pigs with Landrace × Yorkshire pigs increased with the increase of body weight.Correlation analysis showed that in F1 hybrids with boar × Laiwu pigs,the expression of MDH and LPL mRNA had significant positive correlationship with content of intramuscular fat（P0.05）,but had no correlation with rate of polyunsaturated fatty acid to saturated fatty acid（P0.05）.In Landrace × Yorkshire pigs,the expression of MDH and LPL mRNA had no correlationship and had significant positive correlationship with content of intramuscular fat,respectively（P0.05 and P0.05）,and had significant negative correlationship with rate of polyunsaturated fatty acid to saturated fatty acid（P0.01 and P0.05）.The rate of polyunsaturated fatty acid to saturated fatty acid had significant negative correlationship with the content of intramuscular fat in two pig populations（P0.05）.The results suggest that with the increase of body weight,the expression of b

关 键 词：猪 肌内脂肪含量 脂肪酸组成 MDH mRNA LPL 发育性变化 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]