山杏种皮提取物体外抗氧化活性研究  

Antioxidant Activities in Vitro in Testa Extractive of Prunus Armeniacavar.ansu

作　　者：齐高强[1] 赵忠[1] 李巨秀[2] 朱海兰[1] 周锋利[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《中国食品学报》2013年第7期40-45,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家林业局林业公益性科研专项(200904020);科技部科技成果转化资金项目(2009360040128)

摘　　要：目的:研究山杏种皮提取物总多酚含量及其体外抗氧化活性。方法:选用体积分数80%的乙醇为溶剂,采用超声辅助法制备山杏种皮提取物。采用Folin-酚试剂法测定山杏种皮提取物的总多酚含量。采用FRAP法测定山杏种皮提取物的总抗氧化能力。以2,6-二叔丁基对甲酚(BHT)、没食子酸丙酯(PG)为对照样品,分别测定山杏种皮提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、超氧阴离子自由基(O2·軈)、羟自由基(·OH)的清除能力、对β-胡萝卜素/亚油酸体系的抑制能力及其还原力。结果:山杏种皮提取物中总多酚含量为(186.7±15.6)mg/g,其FRAP值为(3.715±0.012)mmol/g;对DPPH·、O2·軈、·OH具有清除作用,清除率分别为80%、97%、98%;对β-胡萝卜素/亚油酸体系具有明显的抑制作用。结论:山杏种皮提取物具有较强的抗氧化能力。Objective： the study was purported to research on the total polyphenol content and in vitro activity of testa extract of wild apricot. Method： testa extract of wild apricot was obtained by ultrasonic-assisted extraction with 80% （V/V） ethanol and its total polyphenol content and total anti-oxidative capacity were assayed with Folin＇s reagent and FRAP, respectively. The capacities of test extract of wild apricot eliminating superoxide anion radical（O2 ）, hydroxyl radical （. OH） and 1, 1 -Diphenyl-2-picrylhydrazyl radical 2, 2-Diphenyl- 1- （2,4,6-trinitrophenyl） hydrazyl （DPPH-） and the capacities of the testa extract inhibiting the fl-carotene/linoleic acid system and reducing were assayed with 2, 6-Ditert-butyl-4-methylphenol（BHT） and proply gallate（PG） as its controls. Results： the testa extract had a total polyphenol content of （186.7＋15.6）mg/g and a FRADP value of （3.715＋0.012）mmol/g, and capable of eliminating superoxide anion radical （O2·）, hydroxyl radical （·OH） and diphenyl bitter acyl radical （DPPH·） at the percentages of 80%, 97% and 98%, respectively and evidently inhibiting the β-carotene/linoleic acid system. Conclusion： testa extract of wild apricot had relatively strong anti-oxidative capacity.

关 键 词：山杏种皮 总多酚 抗氧化活性 自由基清除率 

分 类 号：R151[医药卫生—营养与食品卫生学]