臭柏精油对小鼠的急性毒性试验  

Acute Toxicity Test of Essen Oil of Sabina vulgaris in Mice

作　　者：石晓红[1] 遇艳萍[2] 王林和[3] 张国盛[3] 赵丽芹[1] 包海泉[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]烟台职业学院食品与生化工程系,山东烟台264670 [3]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018

出　　处：《农产品加工（创新版）（中）》2013年第8期17-18,20,共3页Farm Products Processing

基　　金：国家林业公益性行业科研专业项目(201104061)

摘　　要：为了初步评价臭柏精油的毒性，按照改良寇氏法设计试验，选择昆明种小鼠对臭柏精油进行14d的急性毒性试验，观察在14d内动物所产生的毒性反应。试验结束后将小鼠处死解剖，观察心、肝、脾、胃、肾、肺、胸腺等重要器官的病理变化。结果显示，给小鼠灌胃臭柏精油后出现毛发蓬松、运动失调、抽搐、侧卧、呼吸困难、饮水量及采食量减少等症状，小鼠死亡时间集中在12～24h。臭柏精油对小鼠经口急性毒性的LD。为2570mg／kg，95％可信限为2763-2391mg／kg。死亡动物经尸体解剖肉眼观察心、肝、脾、胃、肾、肺、胸腺等重要器官无明显异常。对存活小鼠连续观察14d，一般情况良好，未出现其他毒性反应或死亡。由此可知臭柏精油属低毒物质，但对臭柏精油进行开发利用时应考虑其安全性评价。To evaluate the toxicity of essen oil of Sabina vulgaris to mice, the acute toxicity test is carried out by Karber for 14 days. The mice alive are put to death to observe basic pathologic changes of heart, liver, spleen, stomach, kidney, lung and thymus 14 days later. The mice administrated different dose of esseu oil of Sabina vulgaris show shaggy, movement disorder, twitching, lying on one side, hard breath and reducing of water and food intake. The death time of mice is 12-24 h. The half lethal dose （LD50） of essen oil of Sabina vulgaris is 2 570 mg/kg and its 95% confident_limit is 2 763-2 391 mg/kg. There＇s no other abnormal toxic reactions in basic pathologic changes. These alive mice are observed continuously for 14 days and the resylts show that there is no other toxic effects and death. The results suggest that acute toxicity test from essen oil of Sabina vulgar is low toxic to food safety. However, the safety evaluation is considered in the development of essen oil of Sabina vulgaris.

关 键 词：臭柏精油 急性毒性 小鼠 LD50 

分 类 号：R155[医药卫生—营养与食品卫生学]