豆奶甲醇提取物中抗氧化活性物质的鉴定  

Identification of Antioxidants in Methanol Extracts from Soymilk

作　　者：张华[1] SUMNER Lloyd LEI Zhen-tian HUHMAN David WATSON Bonnie 付福友 于杰[1,4] 

机构地区：[1]西南大学园艺园林学院,重庆北碚400715 [2]The Samuel Roberts Noble Foundation,俄克拉荷马州阿德莫尔市73401 [3]加拿大农业与农业食品部,萨斯卡通SK S7N0X2 [4]南方山地园艺教育部重点实验室,重庆北碚400715

出　　处：《现代食品科技》2013年第8期1999-2003,共5页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(31171930);中央高校基本科研业务费专项资金(XDJK2013A014);西南大学博士启动基金项目(SWU112016)

摘　　要：本文主要在研究了豆奶甲醇提取物有无抗氧化活性的基础上,分析鉴定了其可能存在的抗氧化物质,为相关研究提供理论依据。测定抗氧化能力的方法包括1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl radical,DPPH)法和铁离子还原能力(Ferricreducing antioxidant power,FRAP)法。比色法测定总酚含量和总黄酮含量。通过超高效液相-四极杆飞行时间串联质谱(Ultra-highperformance liquid chromatography-quadruple time-of-flight-mass spectrometry,UHPLC-QTOF-MS)测定豆奶甲醇提取液中活性成分的种类和含量。实验表明,豆奶甲醇提取液具有一定的抗氧化性,且DPPH法和FRAP法测定豆奶抗氧化值不存在显著性差异。豆奶甲醇提取物的总酚含量(285.77±2.93μg/mL)远高于总黄酮含量(11.27±0.51μg/mL)。鉴定出5种化合物,其中只有6,7,4'-三羟基黄酮和Rha-Ara-GlcA-SoyB与抗氧化值存在正相关性。异黄素和大豆甙元两种异黄酮与抗氧化值存在着负相关,6,7,4'-三羟基黄酮可能是主要的抗氧化物质。The paper mainly investigated the antioxidaative capacity of the soymilk methanol extracts and analyzed the possible existing antioxidants. DPPH （1,1-Diphenyl-2-picrylhydrazyl radical） method and FRAP （Ferric reducing antioxidant power ） method were used to analyze the antioxidative capacity of soymilk methanol extracts. The total contents of phenolics and total flavonoids were identified by colorimetric method.The kinds and contents of bioactivities of flavonoids were identified by ultra-high performance liquid chromatography-quadruple time-of-flight-mass spectrometry（ UHPLC- QTOF-MS）.The results showed that the methanol extracts of soymilk owned antioxidative activity. The antioxidative values showed no difference between DPPH and FRAP methods. Total phenolics content （285.77a：2.93 Ixg/mL） was higher than total flavonoids content （11.27i-0.51 ~tg/mL） in soymilk methanol extract. The compounds were identified and quantified by UHPLC- QTOF-MS.Among five bioactivities identified, 6,7,4＇-Trihydroxyflavone and Rha-Ara-GlcA-SoyB showed positive correlation with their antioxidant values. However, genistein and daidzein perform negative correlation with antioxidant values. 6,7,4＇-Trihydroxyflavone may be the main compound attributing to antioxidation of the commercial soymilk.

关 键 词：豆奶 抗氧化性 DPPH法 FRAP法 总酚含量 总黄酮含量 

分 类 号：TS201.2[轻工技术与工程—食品科学]