水溶性纤维素在乳品中的应用及检测方法建立  

The Application of Water-soluble Cellulose in Dairy Products and Establishment of Testing Methods

作　　者：王春霞[1] 昝文汀[1] 刘昊川[1] 梅凡[1] 朱占涛[1] 高云飞[1] 张朝正[1] 李胜元[1] 

机构地区：[1]天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津市工业微生物重点实验室,天津300457

出　　处：《食品研究与开发》2013年第15期62-65,共4页Food Research and Development

基　　金：天津科技大学实验室开放基金项目(1204A302)

摘　　要：在乳制品中添加10 g/L^30 g/L低能量、无糖的特殊碳水化合物-水溶性纤维素,部分地替代糖和脂肪,满足人体对膳食纤维的需求;采用高效液相色谱示差折光检测,以水作流动相,进样量20μL,在流速0.5 mL/min下,直接测定乳制品中的可溶性膳食纤维含量。结果表明:6次平行实验的精密度和稳定性相对标准偏差分别为1.3%和0.74%,回收率在95%~106.3%之间。该方法简单、准确、快捷,适合于乳制品中水溶性膳食纤维含量的测定。In dairy products added 10 g/L-30 g/L low energy and the sugar-free of special carbohydrates, water-soluble cellulose. It can part to alternative sugar and fat, meet the needs of the human body to dietary fiber. Using high performance liquid chromatography with refractive index detection, with water as the mobile phase, into sample volume 20 μL, under the velocity 0.5mL/min, directly determination of the content of soluble dietary fiber in dairy products. Results show that the 6 parallel experimental precision and stability of relative standard deviation 1.3 % and 0.74 %, the recovery rate in 95 %-106.3 %. The method is simple, accurate, fast, suitable for the determination of the content of water-soluble dietary fiber in dairy products.

关 键 词：水溶性纤维素 聚葡萄糖 可溶性膳食纤维 示差折光检测 高效液相色谱 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]