引起天津地区居民口虾蛄过敏的主要致敏蛋白的研究  

Study of the major allergens of edible mantis shrimp among residents of Tianjin

作　　者：陈凯[1] 吴丹[1] 单立新[1] 任杰[2] 

机构地区：[1]天津市北辰医院检验科,天津300400 [2]天津医科大学检验学院,天津300070

出　　处：《中国食品卫生杂志》2013年第5期410-413,共4页Chinese Journal of Food Hygiene

基　　金：天津市北辰区科学技术委员会与卫生局联合资助项目(BCWS2012-06)

摘　　要：目的研究引起天津地区居民口虾蛄过敏的主要致敏蛋白。方法通过皮肤点刺试验收集天津地区116例口虾蛄过敏病人的血清作为特异性探针,与口虾蛄蛋白粗提物进行Western blot试验,鉴定出主要的口虾蛄致敏原。选取5例具有代表性的病人血清为研究对象,以扇贝提取物为抑制剂,进行免疫印迹抑制试验。进而分析以扇贝为代表的软体动物和口虾蛄之间的交叉反应。结果 Western blot试验结果显示,病人血清与不同分子量的蛋白之间有反应,且反应率有差异。与35 kDa的原肌球蛋白的反应率高达64.5%(40/62),与67和>90 kDa的蛋白也有较高的反应率,分别达到41.9%(26/62)和54.8%(34/62)。免疫印迹抑制试验结果显示,原肌球蛋白是扇贝和口虾蛄类的主要交叉反应蛋白,而分子量较大的蛋白几乎不存在交叉反应。结论除原肌球蛋白外,数种大分子量口虾蛄蛋白均可引起天津地区成人对口虾蛄类过敏,且与扇贝的交叉反应率低。这表明一些高分子量的蛋白在口虾蛄类致敏中扮演重要角色。Objective To study the major allergens of edible mantis shrimp among residents of Tianjin.Methods Serums of 116 patients of edible mantis shrimp allergy were collected by skin prick test,and the main allergens were identified from the crude shrimp extract by immunoblotting technique with serums as the probe.Five typical serums were chosen form 62 positive samples.The cross-reactivity between edible mantis shrimp and scallop allergens were tested by immunoblotting inhibition experiments.Results The western blot showed that the serum of allergy pateints had different binding sites.The reaction rate of 35 kDa tropomyosin was 64.5%(40/62),and that of 67 kDa and >90 kDa were 41.9%(26/62) and 54.8%(34/62) respectively.Immunoblotting inhibition showed that the main cross-reaction protein was tropomyosin.The larger molecular weight protein showed less cross-reactivity.Conclusion In addition to tropomyosin,several large molecular weight proteins of edible mantis shrimp could cause allergy among adults in Tianjin and cross-reactivity with scallop was low.This suggested that some of the high-molecular-weight proteins might play an important role in edible mantis shrimp allergy.

关 键 词：免疫印迹试验 食物致敏原 口虾蛄 交叉反应 扇贝 致敏蛋白 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学]