大连地区水产企业加工用水肠球菌检测和分析  

The detection and analysis of Enterococcus in processing water for seafood enterprises in Dalian

作　　者：田卓 麻丽丹 陈晓东 崔妍 闫平平 

机构地区：[1]大连出入境检验检疫局,辽宁大连116000 [2]丹东出入境检验检疫局,辽宁丹东118000

出　　处：《中国食品卫生杂志》2013年第5期461-464,共4页Chinese Journal of Food Hygiene

摘　　要：目的对大连地区水产企业生产加工用水进行肠球菌检测,判断其卫生状况。方法根据水质检测ISO标准(ISO 7899—2∶2000)薄膜过滤法,进行肠球菌分离培养和生化反应鉴定,并根据肠球菌的高度保守的特异性tuf基因,合成了特异性引物进行PCR扩增。结果在41份检测样品中,两种方法的肠球菌检出率均为14.6%,主要为群Ⅱ菌株。结论本地区加工用水卫生状况不理想,应采取行之有效的措施,加强预防和治理。Objective The aim of the study was to investigate the hygiene and sanitation of seafood enterprises according to Enterococcus in processing water.Methods According to the method of ISO7899-2∶ 2000(Water quality-Detection and enumeration of Enterococcus-Part 2: Membrane filtration method),Enterococcus was isolated and identified.Primers and probes were designed and synthesized targeting tuf gene.Results The results showed that 14.6% of the 41 isolates were Enterococcus positive.Most of them were GroupⅡ strains.Conclusion We should take effective measures of prevention and control because the health status was not satisfying.

关 键 词：肠球菌 大连地区 加工用水 食品安全 水产品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]