液相色谱-质谱联用测定乳制品中掺加胶原蛋白的方法研究  

Identification of collagenous protein in dairy products by high performance liquid chromatography-mass spectrometry

作　　者：郑云峰[1] 诸葛庆[1] 曾金红[1] 焦新萍[1] 吴坚[1] 宋海燕[1] 胡建刚[1] 

机构地区：[1]绍兴市质量技术监督检测院国家黄酒产品质量监督检验中心,浙江绍兴312071

出　　处：《中国乳品工业》2013年第9期56-58,共3页China Dairy Industry

基　　金：浙江省质量技术监督系统科研计划项目(20110322)

摘　　要：羟脯氨酸是胶原蛋白的特有成分，通过测定乳制品水解液中的羟脯氨酸来鉴定乳制品中是否掺加了胶原水解蛋白。建立了高效液相色谱-质谱检测羟脯氨酸的方法，采用C8色谱柱，以乙腈-水为流动相，使用电喷雾离子源，正离子检测模式，选择离子方式扫描。结果表明，羟脯氨酸的检出限为1．5mg／kg，在质量浓度为0．1-12mg／L围内呈现良好线性，三个不同添加量的平均回收率为82．0％-91．5％，相对标准偏差为3.3％-8．5％。该方法流动相简单，无需对样品进行衍生，操作简便，定性准确，重现性好，可用于乳制品质量安全的监督检验。Hydrooxyproline is unique component of collagenous protein, this feaature can be used for the identifcafion of coUagenous protein of hydrooxyproline through the determination of dairy products to identify whether the addition of collagenous protein ingredients. This study estabhshed a high performance hquid chromatography-mass spectrometry（HPLC-MS）-method to determinate hydrooxyproline in dairy products which detected positive ionization followed by selected reaction monitoring, hydrooxyproline was separated by the column of C8 with the mobile phase of acetonitrile-water （95：5 ,V/V） . The results showed, when 10-500μg hydrooxyproline was fortified, the average recoveries were between 82.0% and 91.5%, the RSD was between 3.3% and 8.5%, the hmit of determination was 1.5 mg/kg. The calibration curve was linear over a range from 0.1 mg/L to 12 mg/L （R=0.9995）.The method has the advantages of easy operation, good reproducibility and accuracy without derivatization. It can be used for the supervision and inspection of dairy quality and safety.

关 键 词：高效液相色谱-质谱 羟脯氨酸 胶原蛋白 乳制品 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]