复配抑菌剂对假单胞杆菌抑制效果的研究  

Study on the bacteriostatic effect of multiple antimicrobials on Pseudomonas spp

作　　者：靳春秋[1,2] 迟海[1] 杨宪时[1] 李学英[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学食品学院,上海201306

出　　处：《食品工业科技》2013年第20期169-172,184,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(30771675);嘉兴市科技计划项目(SQ2012011194)

摘　　要：在单因素实验的基础上,运用响应面法优化了丁香酚、异硫氰酸烯丙酯、双乙酸钠、壳聚糖4种抑菌剂复配后对假单胞杆菌的抑制效果。结果表明复配抑菌剂最优浓度为:丁香酚0.51g/L、异硫氰酸烯丙酯0.32g/L、双乙酸钠2.24g/L、壳聚糖0.52g/L,在此条件下抑菌率实测值为85.37%,测得最低抑菌浓度为2.59g/L。在此浓度基础上复配抑菌剂对假单胞杆菌的抑菌圈直径达到26.5mm,明显高于茶多酚和苯甲酸钠,表明对假单胞菌杆菌有明显的抑制作用。Based on the single-factor experiments,the responses surface methodology（RSM） were applied to study the effect of eugenol,allylisothiocyanate,sodiumdiacetate,chitosan on the Pseudomonas spp. The result showed that the optimal concentration of ugenol ,allylisothiocyanate, sodiumdiacetate,chitosan was 0.51,0.32, 2.24,0.52g/L, respectively. At this condition the bacteriostatic rate was 85.37%,the minimum inhibitory concentration was 2.59g/L. And the diameter of inhibitionzone was 26.5mm ,significantly higher than the polyphenols and sodium benzoate,the inhibitory effect on the Pseudomonas spp was distinctly.

关 键 词：假单胞杆菌 复配抑菌剂 响应面 抑菌效果 

分 类 号：TS254.3[轻工技术与工程—水产品加工及贮藏工程]