基于免培养法研究泸州地区浓香型白酒窖泥原核微生物群落结构  

Community Structure of Prokaryotes in Pit Mud of Lu-Flavor Liquor from Luzhou Prefecture Based on Culture-independent Approach

作　　者：叶光斌[1,2] 罗惠波[1,2] 杨晓东[1] 李丹宇[1] 王毅[1] 倪斌[3] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000 [3]泸州老窖股份有限公司,四川泸州646000

出　　处：《食品科学》2013年第17期176-181,共6页Food Science

基　　金：泸州老窖奖学金项目(091jzk06);四川理工学院人才引进项目(2010XJKRL001);四川省教育厅重大培育项目(09ZZ015);四川省教育厅重点项目(12ZA099)

摘　　要：通过构建细菌和古菌的16S rRNA基因克隆文库、测序及系统发育树分析,确定浓香型白酒成熟窖泥中细菌和古菌的群落组成。结果表明:窖泥中的细菌多样性丰富,多达34个OTUs。主要分布于14科以及2个科级分类地位未知的梭菌纲和拟杆菌纲类群内,且大多属于严格厌氧细菌。其中梭菌纲为窖泥内细菌群落的绝对优势种群(克隆子数比例为68.2%),除此之外还存在一定比例的芽孢杆菌纲(21.6%)、拟杆菌门(8.0%)、柔膜菌门(1.1%)和Synergistetes门(1.1%)的分布。古菌的群落组成较为简单,仅4个OTUs,主要分布于甲烷囊菌属、甲烷八叠球菌属、甲烷杆菌属和Methanomassiliicoccus内,克隆子数的比例分别为58.8%、29.4%、5.9%和5.9%。The community compositions of bacteria and archaea in mature pit mud of Lu-flavor liquor were detected through establishing 16S rRNA gene clone library of bacteria and archaea, sequencing and analyzing phylogenetic tree. Data analysis showed that the biodiversity of bacteria in pit mud was quite rich, reaching 34 OTUs, which were mainly distributed in 14 families and 2 unidentified families of Clostridia and Bacteroidia. And also, most of them were restricted anaerobes. Among them, Clostridia strains were absolutely dominant in the bacterial community of pit mud, and its ratio of clone number was 68.2%. In addition, there was appropriate distribution for Bacilii （21.6%）, Bacteroidetes （8.0%）, Tenericutes （1.1%） and Synergistetes （1.1%）. Archaeal community composition was quite simple, and only 4 OTUs were detected and mainly distributed in Methanoculleus, Methanosarcina, Methanobacterium and Methanomassiliicoccus with clone distribution ratios of 58.8%, 29.4%, 5.9% and 5.9%, respectively.

关 键 词：浓香型白酒 窖泥 免培养法 细菌 古菌 16S RRNA基因 克隆文库 

分 类 号：Q938.1[生物学—微生物学]