响应面法优化酶解苋籽蛋白制备ACE抑制肽工艺  

Optimization of Enzymatic Hydrolysis of Amaranth Protein for Preparing Angiotensin-Ⅰ Converting Enzyme(ACE) Inhibitory Peptides by Response Surface Methodology

作　　者：陈飞平[1,2] 周家华[2] 常虹[2] 曾凡坤[1] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]北京市农林科学院农业综合发展研究所,北京100097

出　　处：《食品科学》2013年第19期254-260,共7页Food Science

摘　　要：采用响应面法优化碱性蛋白酶酶解苋籽蛋白的工艺条件,制备高活性的血管紧张素转换酶(ACE)抑制肽。通过单因素试验设计,分别考察酶种类、时间、底物质量浓度、酶与底物比、pH值和温度等因素对酶解产物水解度和ACE抑制活性的影响。采用三元二次旋转正交组合设计试验,以ACE抑制率为响应值对工艺进一步优化,经过响应面分析建立回归模型,并从模型中获得最优的酶解条件为:酶与底物比3.50%、pH8.78、温度54.4℃,在此条件下,用碱性蛋白酶在体系底物质量浓度为4g/100mL时酶解苋籽蛋白3h,得到产物的ACE抑制率为46.45%,与预测值46.63%接近,同时其抑制ACE活性的IC50为0.756mg/mL。In the present study, response surface methodology was used to optimize the process conditions for the hydrolysis of amaranth protein by alcalase to prepare highly active angiotensin-I converting enzyme （ACE） inhibitory peptides. One-factorat- a-time designs were used to analyze the effect of enzyme type, hydrolysis time, substrate concentration, enzyme/substrate ratio, hydrolysis pH and hydrolysis temperature on the degree of hydrolysis （DH） and ACE inhibitory activity of amaranth protein hydrolysate. A quadratic regression model describing ACE inhibitory rate as a function of three variables was fitted by using an orthogonal rotary composite design. The optimal hydrolysis conditions were obtained from the regression model as follows： enzyme/substrate ratio of 3.50%, hydrolysis pH of 8.78, hydrolysis temperature of 54.4 ℃, substrate concentration of 4 g/100 mL and hydrolysis time of 3 h. Under these conditions, the ACE inhibitory activity of the obtained hydrolysate was 46.45%, which was close to the predicted value. At the same time, the IC50 of the hydrolysate was 0.756 mg/mL.

关 键 词：籽粒苋 ACE抑制肽 酶解 响应面分析 

分 类 号：TQ420.66[化学工程]