宝塔菜功能性低聚糖体外抗氧化活性及其对体外培养小鼠T淋巴细胞增殖能力的影响  

Antioxidant Activity in vitro of Functional Oligosaccharide from Stachys sieboldii Miq. and Its Effect on the in vitro Proliferation of Mouse Lymphocytes

作　　者：沈德艳[1,2] 郁建平[2] 张训海[3] 王旋[3] 

机构地区：[1]安徽科技学院食品药品学院,安徽凤阳233100 [2]贵州大学生化营养研究所,贵州贵阳550025 [3]家禽疫病防控监测安徽省重点实验室,安徽凤阳233100

出　　处：《食品科学》2013年第19期321-325,共5页Food Science

基　　金：贵州省农业攻关项目(黔科合NY字[2011]3094);安徽高校省级自然科学研究项目(KJ2013Z045);安徽科技学院青年科学基金项目(ZRC2013340)

摘　　要：目的:探讨宝塔菜功能性低聚糖的体外抗氧化活性及其对体外培养小鼠T淋巴细胞增殖能力的影响。方法:以VC为对照,分别采用普鲁士兰法、水杨酸法、邻苯三酚自氧化法以及DPPH法,测定宝塔菜功能性低聚糖的还原力,以及清除羟自由基(·OH)、超氧阴离子自由基(O2-·)和DPPH自由基的能力;采用四甲基偶氮唑蓝(MTT)比色法,测定宝塔菜功能性低聚糖促进外周血淋巴细胞增殖的能力。结果:宝塔菜功能性低聚糖对上述自由基均有不同程度的清除作用,其还原力较低;对·OH和DPPH自由基的清除能力,均在质量浓度小于2.4mg/mL时,弱于VC,当质量浓度为2.4mg/mL时,与VC相当,最高清除率分别达82.0%和89.3%;对O2-·的清除能力弱于VC。宝塔菜功能性低聚糖可以单独或协同ConA刺激T淋巴细胞增殖,且有一定的质量浓度依赖关系。结论:宝塔菜功能性低聚糖具有体外抗氧化和促进外周血T淋巴细胞增殖作用。Objective： To explore the in vitro antioxidant activity of functional oligosaccharide from Stachys sieboldii Miq. and its effect on the in vitro proliferation of mouse lymphocytes. Methods： The reducing power, scavenging capacity against hydroxyl, superoxide anion and DPPH radicals of the functional oligosaccharide and its effect to promote the proliferation of peripheral blood lymphocytes were determined by prussian blue, salicylic acid, pyrogallol autoxidation, DPPH, and methyl thiazolyl tetrazolium colorimetric methods, respectively. Results： The functional oligosaccharide had little reducing power and different scavenging effects on these radical systems. Its scavenging activity against ？OH and DPPH free radical was equal to VC at 2.4 mg/mL and the highest levels were 82.0% and 89.3%, respectively. Its scavenging activity against O2 -· was weaker than that of VC. It could significantly stimulate the proliferation of peripheral blood lymphocytes and showed a synergistic effect with ConA in a dose-dependent manner. Conclusion： The functional oligosaccharide from Stachys seiboibi has antioxidant activity and can promote the proliferation of peripheral blood T lymphocytes.

关 键 词：宝塔菜 功能性低聚糖 体外抗氧化 外周血T淋巴细胞 

分 类 号：TS202.3[轻工技术与工程—食品科学]