二极管阵列-高效液相色谱法同时测定食品中常用的4种食品添加剂  

Simultaneous determination of 4 common additives in food products by a diode array-HPLC system

作　　者：崔鹏[1] 马春光[1] 

机构地区：[1]青海省西宁市疾病预防控制中心,青海西宁810007

出　　处：《现代预防医学》2013年第20期3836-3838,共3页Modern Preventive Medicine

摘　　要：目的建立二极管阵列-高效液相色谱法同时测定食品中安赛蜜、苯甲酸、山梨酸和糖精钠测定方法。方法样品处理后．采用Zorbax Eclipse XDB—C18色谱柱，以甲醇-0．02mol／L乙酸铵溶液（3＋97）为流动相，进行DAD—LC测定。结果在2．5-50μg/ml的线性范围内，呈良好的线性关系，平均回收率在96．9％-103．7％之间，RSD在0．53％～3．72％之间。结论该方法灵敏度高，稳定、准确、重现性好等优点，适用于食品中4种组分添加剂的测定。OBJECTIVE The goal of this study is to establish a simultaneous determination method for detecting acesulfame potassium, benzoic acid, sorbic acid, and sodium saccharin in food products using a diode array-HPLC system. METH- ODS DAD-LC analyses were carried out using a Zorbax Eclipse XDB-C18 column, with methanol-0.02 mol/L of ammonium acetate solution （3＋97） as the mobile phase for the chromatography. RESULTS A good linearity was observed in the range of 2.5 to 50 μg/ml, with an average recovery rate of 96.9 to 103.7% and a RSD of 0.53-3.72%. CONCLUSION The method is sensitive, stable, accurate, and reproducible, and is suitable for determining the contents of the four additives in food products.

关 键 词：二极管阵列 液相 食品 安赛蜜 苯甲酸 山梨酸 糖精钠 

分 类 号：R155.5[医药卫生—营养与食品卫生学]