浓香型大曲中黑曲霉的分离鉴定及其安全性初步研究  

Isolation and Identification of Aspergillus niger Strains from Nong-flavor Daqu and Preliminary Study of Its Safety

作　　者：叶光斌[1] 罗惠波[1,2] 杨晓东[1] 李丹宇[1] 王毅[1] 王彩虹[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《酿酒科技》2013年第10期20-23,共4页Liquor-Making Science & Technology

基　　金：四川省教育厅重大培育项目(09ZZ015);四川理工学院人才引进项目(2010XJKRL001)

摘　　要：采用传统微生物分离手段从浓香型大曲中分离纯化得到菌株NJSYS-6。通过菌落观察、镜检以及分子生物学手段鉴定其为黑曲霉。采用酶联免疫法对其产赭曲霉毒素A进行定量检测,结果表明,在液态发酵进行到第9天时,其赭曲霉毒素A含量达到最高值8.5μg/kg;在固态模拟发酵时,黑曲霉菌株的接种量加大至10‰,蒸馏酒中赭曲霉毒素A含量最高可达0.4μg/kg,丢糟中赭曲霉毒素A含量最高达2.5μg/kg,在国内外标准限制以内。Strain NJSYS-6 was isolated and purified from Nong-fiavor Daqu through traditional microbial isolation method. It was identified as Aspergillus niger through microscopic observation on the colony and molecular biology methods. The quantity of OTA produced by Aspergillus niger was detected by enzyme-linked immunosorbent assays（ELISA）. It was revealed in the experiment that OTA reached the maximum value of 8.5 ixg/kg at the 9 th day of liquid fermentation. For simulated solid fermentation, as the inoculating concentration of Aspergillus niger NJSYS-6 reached up to 10 %0, OTA in distilled liquor reached as high as 0.4 μg/kg, meanwhile OTA in waste distiller＇s grains reached as high as 2.5 μg/kg, which was still within the limits of domestic and oversraseas standards.

关 键 词：浓香型大曲 黑曲霉 ITS 赭曲霉毒素A 酶联免疫法 

分 类 号：TS261.1[轻工技术与工程—发酵工程] TS262.3[轻工技术与工程—食品科学与工程]