添加蜗牛酶对纤维素酶产生菌发酵的影响初探  

Preliminary study on cellulase fermentation with adding snailase by Trichoderma virde

作　　者：张培德[1] 胡琛[1] 余文博 

机构地区：[1]复旦大学生物化学系,上海200433

出　　处：《工业微生物》2000年第4期6-10,共5页Industrial Microbiology

基　　金：国家星火计划项目!( 97B10 176 0 0 0 2 8)资助课题

摘　　要：从上海市郊农村草堆的土壤中分离到一株分解纤维素的菌 ,经初步鉴定为绿色木霉。该菌最适生长温度为 2 8℃。在本实验条件下 ,该菌的产酶高峰在 96h左右 ;对木薯渣的水解能力最强 ,滤纸的水解活力 (FilterPaperActivity ,FPA)为 1 0 .8u/ml发酵液。其酶的最适反应温度为 5 0℃ ,最适pH为 4.8。用添加蜗牛酶的方法进行绿色木霉菌发酵 ,结果显示 ,分泌纤维素酶的能力提高 1 2 %左右。A strain was screened from the soil of Shanghai suburb, and was identified as Tichoderma virde . The experiment indicated that the optimum growth temperature of the strain was 28～30℃ and the optimum time of cellulase secreted was about 96 hrs. The hydrolytic level (the Filter Paper Activity, FPA) of cassava dregs was 10.8u/ml broth. The optimum conditions for cellulase action was at pH 4.8, 50℃. The fermentation yield of Trichoderma virde was increased by about 12% after adding snailase 500～800mg/L broth.

关 键 词：纤维素酶 蜗牛酶 绿色木霉 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]