毛霉高产中性蛋白酶发酵培养条件的优化  

Optimum Culture Conditions for High-yielding Neutral Protease of Mucor

作　　者：刘颖[1] 孙冰玉[1] 刘琳琳[1] 刘莹莹[1] 张光[1] 石彦国[1] 

机构地区：[1]哈尔滨商业大学食品工程学院/黑龙江省普通高等学校食品科学与工程重点实验室,哈尔滨150076

出　　处：《大豆科技》2013年第5期33-39,共7页Soybean Science & Technology

基　　金：十二五国家科技支撑计划项目"固态发酵传统大豆食品品质提升关键技术及新产品开发"(2012BAD34B03-4)

摘　　要：为了提高毛霉产中性蛋白酶酶活力,通过单因素实验和正交实验对毛霉发酵培养条件进行优化,结果表明,雅致放射毛霉产中性蛋白酶最佳培养条件为:培养时间72 h、培养温度30℃、pH6.8、接种量9%,此条件下中性蛋白酶酶活力达1 967.36 U/mL;五通桥毛霉产中性蛋白酶最佳培养条件为:培养温度27℃、培养时间78 h、pH6.5、接种量7%,此条件下中性蛋白酶酶活力达1 896.53 U/mL。In order to improve the neutral protease activity of mucor, optimization of culture condition during mucor fermentation was examined. Based on the single-factor experiment and orthogonal experiment, the optimized culture conditions were obtained. For the neutral protease production of Actinomucor elegans, the incubation time was 72 h, incubation temperature was 30 ℃, pH6.8 and the amount of mucor inoculated spores was 9%, the neutral protease activity was 1 967. 36 U/mL; For the neutral protease production of Wutungkiao Mucor, the culture temperature was 27 ℃, incubation time was 78 h, pH6.5 and the amount of mucor inoculated spores was 7% with protease activity of 1 896.53 U/mL.

关 键 词：毛霉 培养条件 优化 中性蛋白酶酶活力 

分 类 号：TS201.3[轻工技术与工程—食品科学]