膳食中晚期糖基化终产物对大鼠肾脏的影响  

Effect of Dietary Advanced Glycation End Products on Diabetic Rat Kidney

作　　者：张双凤[1] 苏亚儒[1] 李巨秀[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2013年第21期315-320,共6页Food Science

基　　金：国家自然科学基金项目(31101217);西北农林科技大学科技创新专项(QN 2012034)

摘　　要：以SD大鼠为实验动物,建立糖尿病大鼠模型,用含量不同的晚期糖基化终产物(AGEs)饲料饲喂糖尿病鼠和正常鼠8周,研究大鼠肾脏系数、血肌酐、血清荧光值、肾组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的变化,观察AGEs摄入对大鼠肾脏的影响,并对其机制进行探讨,以评估膳食中AGEs的安全性。结果表明:AIN-93G饲料经过高温烘烤(125℃,3h),荧光性AGEs增加4倍左右。从第4周到第8周,AGEs实验组大鼠的肾脏损伤加剧,大鼠肾脏氧化应激加剧。喂养至第8周,与低AGEs饲料糖尿病鼠组相比,高AGEs饲料糖尿病鼠组的肾脏系数((14.84±2.09)g/kg)、血肌酐浓度((40.55±3.51)mol/L)、血清荧光值((898.60±58.07)AU)、MDA含量((5.05±0.42)nmol/mgpro)均显著升高(P<0.05)、SOD活力((89.11±11.07)U/mgpro)、GSH含量((4.12±0.58)mg/gpro)均降低,但差异不显著(P>0.05);与低AGEs饲料正常鼠组相比,高AGEs饲料正常鼠组的肾脏系数、血肌酐浓度、血清荧光值、MDA含量分别上升了11.86%、31.76%、44.49%、163.02%,SOD活力、GSH含量分别下降了50.56%、65.23%。因此,高AGEs饲料促进糖尿病鼠和正常鼠的肾功能恶化,作用机制可能与氧化应激有关。限制AGEs的摄入,可以作为延缓糖尿病肾病恶化的措施之一。48 SD rats were adopted as experimental animals to establish diabetic rat models using streptozotocin Kidney weight / body mass index, serum creatinine , serum fluorescence , kidney tissue superoxide dismutase （SOD）, malondialdehyde （MDA）, and reduced glutathione （GSH） changes in rat were evaluated after feeding with different content of advanced glycation products （Advanced glycation end products, AGEs） for 8 weeks , Thus,The effect and mechanism of diet rich in AGEs on rat kidney were investigated to evaluate the safety of AGEs in diet . The results showed that： Fluorescent AGEs of AIN-93G diet increased about 4 times after baking at high temperatures （125 ℃, 3h）.Effect of AGEs on the rat kidney deepened along with time and oxidative stress from 4 to 8 weeks.8 weeks later, compared with low AGEs diet diabetic rats , kidney weight / body mass index （14.84 ± 2.09g/kg）, serum creatinine concentration （40.55 ± 3.51μmol / L）, serum fluorescence values（898.6 ± 58.07AU） and MDA content （5.05 ± 0.42nmol/mgprot） of high AGEs diet diabetic rat were significantly higher （p 0.05）; compared with low AGEs normal rats, kidney weight / body mass index, serum creatinine concentration, serum fluorescence values and MDA content of high AGEs normal rats increased 11.86%, 31.76%, 44.49% and 163.02%,respectively,while SOD activity and MDA content decreased 50.56% and 65.23%,separately. In summary So high AGEs in diet promote deterioration of renal function in rats and its mechanism may be associated with oxidative stress. Limiting the intake of AGEs can be introduced as one of the measures to slow deterioration of diabetic nephropathy.

关 键 词：晚期糖基化终产物 糖尿病 肾病 氧化应激 

分 类 号：TS201.2[轻工技术与工程—食品科学]