沙棘籽酶解多肽的抗氧化性能研究  

Study on antioxidant activity of peptide fractions from seabuckthorm seed enzymatic hydrolysates

作　　者：连伟帅[1] 王常青[1] 于书佳[1] 原敏[1] 訾艳[1] 陈晓萌[1] 李小凡[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《食品工业科技》2013年第22期128-130,140,共4页Science and Technology of Food Industry

摘　　要：目的:研究沙棘籽三种蛋白酶水解多肽的抗氧化效果。方法:对三种蛋白酶的水解多肽进行体外清除自由基和总抗氧能力的实验及筛选,选取效果最好的一种进行体内抗氧化实验。结果:在胃蛋白酶、胰蛋白酶和木瓜蛋白酶水解沙棘籽得到的多肽中,胃蛋白酶水解的沙棘籽多肽的综合抗氧化结果最佳;动物实验表明,胃蛋白酶水解多肽灌胃的小鼠血液中GSH、CAT等抗氧化指标与阳性组相比差异显著(p<0.05),MDA指标降低效果显著(p<0.05)。结论:胃蛋白酶水解的沙棘多肽与其他两种多肽相比具有更好的抗氧化效果。Objective：There proteolytic enzymes,including pepsin,trypsin,papain and pepsin,were employed to hydroyze Seabuckthom seeds protein to produce the hydrolysates of antioxidant activity. Methods..The radical scavenging capacities and total antioxidant capacities in vitro antioxidant trials of three proteolytic peptides were compared and screened. Selected the best poiypeptide from vitro antioxidant trials to do the oxidation experiment in vivo. Results ：Among the three protein,the seabuckthorn seeds protein hydrolyzed produced by pepsin,had the highest antioxidangt activity. Result of animal oxidation experiment showed that antioxidant indicators of which was GSH and CAT were extremely significant（p〈0.05） compared with the model group,that was seabuckthorn seed polypeptide hydrolyzed by pepsin. And lowering effect of the MDA indicators was significant（p〈0.05） compared with the model group. Conclusion：There was a better antioxidant in hydrolysis seabuckthorn poiypeptide by pepsin,compared with the other two polypeptides.

关 键 词：沙棘籽多肽 不同蛋白酶 抗氧化 动物实验 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]