低次烟叶多糖的提取及生物活性分析  

Extraction and Biological Activity Analysis of Polysaccharides from Low Grade Tobacco

作　　者：许春平[1] 杨琛琛[1] 王铮[1] 张军涛[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,郑州市东风路5号450002

出　　处：《烟草科技》2013年第11期52-55,共4页Tobacco Science & Technology

基　　金：河南省"百人计划"资助项目(豫组函[2012]12号)

摘　　要：为优化低次烟叶多糖的提取工艺、研究其生物活性,通过单因素试验和L9(34)正交试验对低次烟叶多糖提取的超声波功率、提取时间、料液比和提取温度进行了优化,利用Sepharose CL-6B羧甲基琼脂糖凝胶柱对得到的粗多糖进行了分离纯化,考察了低次烟叶多糖对·OH自由基、DPPH·自由基的清除能力,并测定了多糖的体外总抗氧化能力。结果表明:①确定的最佳提取条件为:超声波功率600 W、时间4 min、料液比1∶25和温度60℃。在此条件下,多糖得率为2.56%。②低次烟叶多糖由两种不同分子量的组分组成。③低次烟叶多糖具有较强的总抗氧化活性和较好的自由基清除活性,具有潜在的营养保健作用。In order to optimize the technology for extracting polysaccharides from low graae tooacco anu study their bioactivity, four factors, including ultrasonic power, extraction time, extraction temperature and solid/liquid ratio, were optimized with single factor test and L9（34） orthogonal test. The obtained crude polysaccharides were purified with a Sepharose CL-6B carboxymethyl agarose gel column, the scavenging activity of polysaccharides to free-radicals ＂OH and DPPH. was investigated, and the general determined in vitro with ABTS method. The results showed that antioxidant activity of polysaccharides was l） The optimal conditions were： ultrasonic power 600 W, solid/liquid ratio 1：25, and extracting for 4 minutes at 60 ~C. Under such conditions, the yield of polysaccharides was 2.56%. 2） The obtained polysaccharides contained two fractions of different molecular weights. 3） The polysaccharides possessed strong in vitro antioxidant activity, obvious free-radical scavenging activity and potential nourishing and health care function. The results provided certain theoretical references for the utilization of low grade tobacco.

关 键 词：低次烟叶 多糖 超声波辅助提取 抗氧化活性 

分 类 号：TS411.1[农业科学—烟草工业]