茯砖茶中降解3-苯氧基苯甲酸的“金花菌”筛选及其降解特性  

Screening and identification of 3-phenoxybenzoate acid degrading “Gloden Flower Fungi” from Fuzhuan tea and its degradation characteristic

作　　者：李金永[1] 刘书亮[1] 姚开[2] 李建龙[1] 

机构地区：[1]四川农业大学食品学院,硕士研究生四川雅安625014 [2]四川大学轻纺与食品学院,四川成都610065

出　　处：《食品与发酵工业》2013年第10期45-51,共7页Food and Fermentation Industries

基　　金：特定益生菌降解食品中拟除虫菊酯类农药中间产物-3-苯氧基苯甲酸的特性及其机理研究(31371775)

摘　　要：以茯砖茶为菌源，分离筛选出20株具有3-苯氧基苯甲酸（3-PBA）降解能力的“金花菌”，其中分离的菌株ET1可在7d内完全降解PD培养基中100mg／L的3-PBA，根据其形态特征和ITS序列分析（Gen Bank Accession No．KF317836），将其鉴定为冠突散囊菌（Eurotiumcristatum）。动力学研究表明，菌株ET1降解3-PBA的过程符合一级动力学方程，在测试的3-PBA质量浓度、温度、pH值范围内，3-PBA半衰期为1．7～3．2d，远远低于其自然降解半衰期（180d），且该反应的速率受pH值影响较大，受3-PBA质量浓度和温度的影响相对较小。Adopting fuzhuan tea as bacteria source, twenty strains of 3-phenoxybenzoate acid （3-PBA） degrading ＂golden flower fungi＂ were screened. Strain ET1 isolated from the fuzhuan tea could completely degrade 100mg/L 3- PBA in the PD medium in 7d. Based on the analysis of morphological characteristics and its sequence （ GenBank Ac- cession No. KF317836 ） , strain ET1 was identified as Eurotium cristatum. Kinetic research indicated that the degra- dation of 3-PBA by strain ET1 could be described by a first-order kinetics model. The degradation half-life of 3-PBA was 1.7 to 3.2d over a certain range of 3-PBA concentration, temperature and pH, which was much shorter than its natural half-life. The pH were found to influence the reaction rate obviously but the concentration of 3-PBA and temperature had little effect on reaction rate.

关 键 词：3-苯氧基苯甲酸 微生物降解 “金花菌” 冠突散囊菌 降解特性 

分 类 号：TS272[农业科学—茶叶生产加工]