充氮蒸馏-盐酸副玫瑰苯胺比色法测定蔬菜及其制品中的亚硫酸盐  

Determination of Sulfite in Vegetables and Derived Products by Nitrogen Distillation-Pararosaniline Hydrochloride Spectrophotometric Method

作　　者：方长云[1] 段彬伍[1] 孙成效[1] 卢林[1] 何小嫣[1] 

机构地区：[1]中国水稻研究所,农业部稻米及制品质量监督检验测试中心,浙江杭州310006

出　　处：《食品科学》2013年第22期213-217,共5页Food Science

基　　金：农业部农业行业标准制定项目(农财政[2011]53号)

摘　　要：采用改良充氮蒸馏法提取、甲醛溶液吸收一盐酸副玫瑰苯胺比色测定蔬菜及其制品中的亚硫酸盐含量。结果表明：当称样量为5．00g时，该方法最小检出量为0．5mg／kg，亚硫酸盐含量在0～10μg范围内与吸光度呈良好的线性关系（R2=0．9998）；方法精密度为0．75％-3．78％，平均回收率为89．8％-98．3％。该方法具有可排除颜色干扰、减少有毒试剂的使用和对环境的污染等优点，且方法操作简便、重复性好，符合检测蔬菜及其制品的需求。A nitrogen distillation-formaldehyde solution absorption-pararosaniline hydrochloride spectrophotometric method for the determination of sulfite in vegetables and derived products was developed. The results showed that the detection limit for sulfite was 0.5 mg/kg in 5.0 g of samples. This method had a good linearity （R2 = 0.9998） for sulfur dioxide with a concentration ranging from 0 to 10 μg. The relative standard deviation of the proposed method ranged from 0.75% to 3.78%. The recovery rate was in the range of 89.8% - 98.3%, The developed method has less color interference from samples and less pollution. It is easy and simple with good repeatability, which is suitable for the determination of sulfite in vegetables and derived products.

关 键 词：盐酸副玫瑰苯胺 蔬菜及其制品 亚硫酸盐 最小检出量 精密度 

分 类 号：TS207.5[轻工技术与工程—食品科学]