毕赤酵母表达系统研究进展  

Advances of Pichia pastoris expression system

作　　者：马银鹏[1] 王玉文[1] 党阿丽[1] 孔祥辉[1,2] 张介驰[1,2] 

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150010 [2]黑龙江省科学院高技术研究院,哈尔滨150020

出　　处：《黑龙江科学》2013年第9期27-31,共5页Heilongjiang Science

基　　金：哈尔滨市创新人才研究专项资金项目(2011RFQYS112);黑龙江省科学院基金项目(KW12B01)

摘　　要：毕赤酵母是外源蛋白表达的一种重要宿主。毕赤酵母表达系统既具有原核表达系统操作简单、价格低廉、生产率高的优点,又具有真核表达系统能对表达后的蛋白折叠、糖基化和形成二硫键等翻译后进行加工和修饰的功能,因此毕赤酵母表达系统具有广泛的应用前景和重要的研究价值。本研究对毕赤酵母表达系统的特点、组成、影响外源基因高效表达的影响因素等进行总结。The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production. P. pastoris is easy to manipulate,less expensive,high producing rate of heterogenous proteins as procaryotic organism.Meanwhile,it is also a eukaryote and capable of many posttranslational modifications performed by higher eukaryotic cells,such as correct folding,O- and N- linked glycosylation,disulfide bridge formation. P. pastoris system has demonstrated it＇s applications and values in commerical and research labs. In the view,the characters,constitutions,and influencing factors of efficient expression of P. pastoris system were summarized.

关 键 词：毕赤酵母 表达系统 外源蛋白 甲醇 

分 类 号：Q815[生物学—生物工程]