杏酒中有机酸类化合物的RP-HPLC法测定  

Determination of organic acids compounds in abricotine by reversed-phase high performance liquid chromatography

作　　者：贺小贤[1] 彭光杰[1] 郑茂奎 赵会芳[1] 刘昌蒙[1] 孙福林[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2013年第6期124-127,135,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省教育厅自然科学基金项目(11JK0638);西安市科技计划项目(NC1120(3));咸阳市科技计划项目(2010K05-03)

摘　　要：建立了反相高效液相色谱测定杏酒中有机酸类化合物的方法.以金太阳杏为原料,经液体发酵法精心酿制成的低度杏酒作为检测样品,测定了杏酒中9种有机酸类化合物.采用Agilent C18色谱柱(4.6mm×250mm)分离,流动相2%的甲醇-0.01mol/L(NH4)2HPO4(pH 2.8)洗脱,流速1mL/min,检测器波长为210nm,柱温30℃,采用色谱峰保留时间定性,面积外标法定量,并比较了不同杏发酵产品中有机酸的种类和含量,各成分的线性相关系数均大于0.99.方法的回收率大部分都在94%以上,RSD均在3%以下,说明该方法具有快速、分辨率高、重复性好等优点,可用于杏酒中有机酸类化合物的检测.RP HPLC（reversed phase high performance liquid chromatography） method for the determination of organic acids compounds in apricot wine was developed, l.ow-alcohol apricot wine as samples which was brewed by liquid fermentation with Gold sun apricot, 9 kinds of organic compounds in the apricot wine were determined by retention time of chromatographic peak. The relative determination were summed up as follows： Agilent HC-C18 chromato graphic column （4. 6 mm × 250 mm）, mobile phase is 2% methanol-0. 01 mol/L （NH4）2HPO4（pH2.8）, the flow rate of 1 mL/min, detector wavelength of 210 nm, 30 ℃ column temperature. Area external standard method as quantitative method, and compared the type and contents of organic acids in different apricot fermented products. I.inear correla tion coefficient of each component were greater than 0.99. The recoveries were above 94% in method, and RSD were below 3%. The experiments results show that the RP-HPLC method has the advantages of rapid, high resolution, good repeatability and can be used for analysis of organic acids compounds in apricot wine.

关 键 词：反相高效液相 杏酒 有机酸类化合物 

分 类 号：TS261.7[轻工技术与工程—发酵工程]