油菜蜂花粉多酚超声波提取及抗氧化活性  

Ultrasonic Extraction and Antioxidant Activity of Polyphenols from Rape Bee Pollen

作　　者：龚明贵[1] 张巧明[2] 尤晓颜[1] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南科技大学林学院,河南洛阳471003

出　　处：《食品研究与开发》2013年第19期20-23,共4页Food Research and Development

基　　金：河南科技大学博士启动基金项目(09001704);国家自然科学基金青年项目(C010201)

摘　　要：使用超声波提取,Folin-Ciocalteu比色法测定总酚含量,以多酚提取率为考察指标,通过单因素和正交试验优化油菜蜂花粉多酚的最佳提取工艺;并通过清除羟自由基、亚硝酸根离子、DPPH和ABTS+·自由基能力测定,对油菜蜂花粉多酚的体外抗氧化活性进行评估。结果表明:油菜蜂花粉多酚的最佳提取工艺条件为60%乙醇作提取液、料液比1∶9(g/mL)、以固定功率(500 W)超声波提取3次、每次25 min,油菜蜂花粉多酚的得率为1.652%(即16.25 mg/g);在试验一定质量浓度范围内,油菜蜂花粉多酚对羟自由基、亚硝酸根离子、DPPH和ABTS+·自由基的最大清除率分别为90.34%、87.46%、67.45%和80.12%,具有较好的抗氧化作用。The ultrasonic extraction of polyphenols from rape bee pollen was optimized by single factor and orthogonal tests in order to maximize the extraction efficiency.The vitro antioxidant activities of polyphenols against nitrite ions,hydroxyl,DPPH and ABTS＋·free radicals were also determinated the ability to scavenge The results indicated that 60 % ethanol as extraction solvent at a solid-to-liquid ratio of 1∶9（g/mL） for three repeated extraction for 25 min each time were the optimal extraction conditions,and the highest yield of polyphenols was 1.652 %（16.25 mg/g）under the above conditions.The maximum scavenging rates against nitrite ions,hydroxyl,DPPH and ABTS＋·free radicals were 87.46 %,90.34 %,67.45 % and 80.12 %,respectively.

关 键 词：油菜蜂花粉 超声波提取 多酚 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285[轻工技术与工程—食品科学与工程]