大孔吸附树脂分离纯化红小豆多酚工艺及效果  

Technology of separation and purification and its efficiency of adzuki bean polyphenols with macroporous adsorption resins

作　　者：陶莎[1] 黄英[1] 康玉凡[2] 辰巳英三[3] 张惠[1] 薛文通[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业大学农学与生物技术学院,北京100083 [3]日本国际农林水产业研究中心

出　　处：《农业工程学报》2013年第23期276-285,共10页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家现代农业产业技术体系(CARS-09-G15)

摘　　要：为开发利用红小豆加工副产物中的生理活性物质,该研究采用大孔树脂吸附法对煮制红小豆水的多酚类物质进行分离纯化,比较了5种不同型号大孔树脂对红小豆多酚的吸附分离效果,从中筛选出HPD 600型树脂作为理想的吸附剂;研究了HPD 600树脂对红小豆多酚的吸附等温线,结果表明,该吸附等温线与Langmuir、Freundlich函数曲线的拟合程度非常高,且采用Langmuir模型的拟合效果略优于Freundlich模型。静态和动态吸附、洗脱试验结果表明:样品液浓度、温度、pH值、乙醇浓度、上样流速及洗脱流速等因素均对HPD 600树脂吸附分离红小豆多酚有影响。较理想的工艺参数为:30℃是较适宜的静态吸附温度;保持煮制红小豆水本身的多酚浓度0.96 mg/mL和pH值6.8,上样体积200 mL,上样流速1.0 mL/min进行动态吸附;吸附饱和平衡后,采用50 mL 60%乙醇溶液,以1.5 mL/min的流速进行动态洗脱。依此得到的红小豆多酚纯化液,其总酚含量和总抗氧化能力均有显著提高,约为纯化前的2.2倍。In the process of adzuki bean paste production, lots of wastewater isproduced. The cost of traditional anaerobic wastewater treatment is high, and the materials rich in adzuki bean water can＇t be fully used, such as polyphenols. It is also not good for enterprises to increase product added value. This paper focuses on the separation and purification technology of adzuki bean polyphenols. The adzuki bean polyphenols were purified using a macroporous adsorption resins method. By comparing the adzuki bean polyphenols separation effect of five different types（S-8,NKA-9、AB-8、D4020, and HPD 600） of macroporous resin, HPD 600 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate. The adsorption isotherm of HPD 600 resin was studied, and the results showed that with the increase of polyphenols equilibrium concentration, the adsorptive amount of HPD 600 increased gradually. After the polyphenols concentration increased to a certain degree, the adsorption amount growth trend gradually smoothed. Through a nonlinear curve fitting with the Langmuir and Freundlich adsorption models, it was found that the fitting degree of adsorption isotherm and Langmuir and Freundlich function curve was very high, and the correlation coefficient R2was greater than 0.99. The fitting effect of the Langmuir model（R2=0.9986） was slightly better than the Freundlich model（R2= 0.9943）. Static and dynamic adsorption, and desorption experiment results indicated that sample concentration, temperature, pH value, ethanol concentration, sample flow velocity, and desorption flow velocity had great influence on macroporous resin in the process of adsorbing and separating adzuki bean polyphenols. The ideal technological parameters were keeping the temperature for 30℃, the original concentration and pH value of hot water extracts of adzuki beans, sample volume 200 mL, flow velocity 1.0 mL/min until reaching adsorption balance, dynamically elute with 50 mL 60% ethanol, and the desorption flow v

关 键 词：树脂 吸附 工艺 红小豆水 多酚 抗氧化作用 

分 类 号：TS201.1[轻工技术与工程—食品科学]