反相高效液相色谱法测定秋葵干蔬中的β-胡萝卜素  

Determination of β-carotene in dry okra by reverse phase high performance liquid chromatography

作　　者：董爱军[1] 杨鑫[1] 张英春[1] 张华[1] 井晶[1] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090

出　　处：《食品工业科技》2013年第24期64-66,69,共4页Science and Technology of Food Industry

摘　　要：建立了测定秋葵干蔬中β-胡萝卜紊的高效液相色谱法。样品皂化后，采用反相高效液相色谱-紫外可见检测器进行测定，外标法定量。色谱柱为C18柱（250mm×4．6mm，i．d．5μm），流动相为乙腈-甲醇-乙酸乙酯（88：10：2），流速1mL／min，检测波长453nm。结果表明，β-胡萝卜素在0．1-10．0mg／L浓度范围内呈现良好的线性关系，相关系数为0．996；β-胡萝卜素的回收率为82．4％～94．0％，相对标准偏差（RSD）为2．28％-5．43％；秋葵干蔬中β-胡萝卜素的检出限（LOD）为0．08mg／kg，定量下限（LOQ）为0．26mg／kg。本方法的准确度和精密度都很高，能够满足实际测定需要。Okra was a popular health food in the world,and it could be used for many purposes,such as a vegetable,soup ingredient and fat substitute in chocolate. In this paper,a method was developed for the determination of β-carotene in dried okra by reverse phase high performance liquid chromatography（RP- HPLC）. After the sample was extracted with ethanol,the mixture was saponified at room temperature overnight, and then the saponification liquid was extracted with n-hexane. The extract was separated on a C18 column （250mm×4.6mm,5μm） at 30℃,which was eluted with acetonitrile-methyl alcohol-ethyl acetate（88：10：2,v：v：v） at a rate of lmL/min,and the signal was detected at 453nm. The results showed that：β-carotene was linear over the concentration range of 0.1-10.0mg/L with a correlation coefficient of 0.996 ;the recovery of β-carotene in dried okra was 82.4%-94.0%,the relative standard deviations（RSD） was 2.28%-5.43%,the limit of detection （LOD） of β-carotene in dried okra was 0.08mg/kg,the limit of quantification（LOQ） was 0.26mg/kg. This method was reliable and accurate,and it could meet the demands of actual measurement.

关 键 词：反相高效液相色谱 Β-胡萝卜素 秋葵干蔬 

分 类 号：TS207.3[轻工技术与工程—食品科学]