黑曲霉TNA-09中α-葡萄糖苷酶基因敲除的研究  

Alpha-glucosidase gene knockout of Aspergillus niger TNA-09

作　　者：王露[1] 杨赢[1] 刘燕[1] 王亚军[1] 杨建松[1] 高年发[1] 王德培[1,2] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市工业微生物重点实验室,天津300457

出　　处：《中国酿造》2013年第11期27-31,共5页China Brewing

基　　金：科技部高科技发展研究计划(863项目)(2011AA02A205)

摘　　要：以柠檬酸生产菌黑曲霉TNA-09为原始菌株,通过农杆菌介导的方法,将其α-葡萄糖苷酶基因敲除,得到基因敲除菌株TGA101。对原始菌株黑曲霉TNA-09和基因敲除菌TGA101α-葡萄糖苷酶活力测定,结果相对于TNA-09菌株,TGA101菌株α-葡萄糖苷酶活力下降61.15%。在柠檬酸发酵试验中表现出相对于黑曲霉TNA-09,TGA101菌株发酵液中异麦芽糖含量降低84.67%,柠檬酸提高2.34%,糖酸转化率提高2.34%。In this thesis, α-glucosidase gene of the original strain Aspergillus niger TNA-09 was knockouted by Agrobacterium-mediated method in citric acid production, and knockout strain TGA101 was obtained, α-glucosidase activity of gene knockout strain TGA101 was detected, which decreased by 61.15% than original strain of A. niger TNA-09. It was showed in the citric acid fermentation experiments, compared to A. niger TNA-09, TGA 101 fermentation liquid isomaltose decreased by 84.67%, citric acid increased by 2.34%, sugar/acid conversion rate increased by 2.34%.

关 键 词：黑曲霉TNA-09 Α-葡萄糖苷酶 异麦芽糖 基因敲除 柠檬酸 

分 类 号：TQ925[轻工技术与工程—发酵工程]