菌种对苹果渣发酵饲料中蛋白酶活、纤维素酶活及总酚含量的影响  

The effect of the strains on proteinase activity,cellulase activity and total phenolic content in apple pomace fermented feed

作　　者：张高波[1] 李巨秀[1] 来航线[2] 彭智超 何亚军[1] 卫伟[1] 程方[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《食品与发酵工业》2013年第11期118-123,共6页Food and Fermentation Industries

基　　金："十二五"国家科技支撑计划项目(2012BAD14B11)

摘　　要：研究了发酵菌种对苹果渣发酵饲料中蛋白酶活、纤维素酶活以及总酚含量的影响。以未接菌的固态发酵培养基作为空白对照,通过单一菌种、双菌组合、菌种比例发酵试验,采用比色法对发酵产物进行蛋白酶活、纤维素酶活及总酚含量分析。结果表明:产朊假丝酵母HJ1和黑曲霉HF3组合是优选菌种配伍,接菌比例为1∶1时,发酵产物中蛋白酶活(742.40±56.77)U/g,纤维素酶活(64.31±3.19)U/g,总酚含量达(5.89±0.47)g/kg,与对照组相比分别增加了207.32%,947.45%和86.00%。适宜菌种比例的双菌发酵对于提高苹果渣发酵饲料中蛋白酶活、纤维素酶活以及总酚含量有显著作用(P<0.01)。In this experiment,the strains of solid state fermentation,which would affect the proteinase activity,cellulase activity and total phenolic content of apple pomace fermented feed,was studied to provide the scientific basis for the production of apple pomace active protein feed. Solid state fermentation medium without inoculation was used as control. The effects of single-strain fermentation and mixed-strains fermentation of apple pomace on proteinase activity,cellulose activity and total phenolic content were analyzed by colorimetric spectroscopy. The results showed that,the combination of Aspergillus niger HF3 and Candida utilis HJ1 is the optimal strain combination. When inoculated by Aspergillus niger HF3 and Candida utilis at the ratio of 1∶ 1,the proteinase activity,cellulase activity and total phenolic content reached（ 742. 40 ± 56. 77） U / g,（ 64. 31 ± 3. 19） U / g and（ 5. 89 ± 0. 47） g / kg,which were increased by 207. 32%,947. 45%,and 86. 00%,respectively,compared with blank samples. The proteinase activity,cellulase activity and total phenolic content of apple pomace fermented feed had been obviously improved by mixed-strains fermentated treatment（ P 0. 01）.

关 键 词：苹果渣 菌种 发酵饲料 蛋白酶活 纤维素酶活 总酚含量 

分 类 号：S816.6[农业科学—饲料科学]