基于核磁共振扫描(NMR)的赭曲霉毒素A毒性血浆代谢组学研究  

Study on Toxicity of Ochratoxin A by Nuclear Magnetic Resonance (NMR) Based Metabolomics

作　　者：夏凯[1] 贺晓云[1] 郝俊冉[1,2] 黄昆仑[1,2,3] 梁志宏[1,2] 许文涛[1,2,3] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]农业部转基因生物食品安全检验监督测试中心,北京100083 [3]营养健康与食品安全北京市重点实验室,北京100083

出　　处：《农业生物技术学报》2013年第12期1426-1433,共8页Journal of Agricultural Biotechnology

基　　金：北京市科技计划项目(No.D121100003112001;No.D121100003112004)

摘　　要：赭曲霉毒素A(OTA)是由某些真菌产生的次级代谢产物,广泛分布于谷物、酒类以及肉类等食品中。前人研究表明,OTA对啮齿类动物和人体具有肾脏毒性、肝脏毒性,以及致畸、致突变和潜在致癌等毒性。为了探究OTA在代谢水平产生的毒害作用,本研究以氢核磁共振谱(1H NMR)作为主要研究平台结合病理组织分析、血生化分析等实验手段在代谢水平研究了OTA的毒性。结果表明,OTA对大鼠(Rat tus norvegicus)肾脏具有毒害作用。病理切片结果显示,OTA诱导肾脏外髓质表皮细胞的细胞质空泡化和肾近端小管表皮细胞的核巨大化;1H NMR结果显示,血浆中乳酸、苏氨酸、脂质以及高密度脂蛋白等化合物含量发生变化,说明OTA改变了大鼠的基础代谢,并影响了糖代谢和脂代谢的相关代谢通路。这一发现为从代谢组学的角度深入理解OTA的致毒机制提供了有价值的信息。Ochratoxin A（OTA） is a mycotoxin produced by several kinds of fungi growing on food source materials. OTA has been proved to be nephrotoxic, hepatotoxic, teratogenic, mutagenic and potential carcinogenic etc. to rodents and even human. In this paper, the toxicity of OTA was studied through 1H NMR（nuclear magnetic resonance） based metabolomics together with serum clinical analysis and histopathological examination. The results showed that OTA induced the damages in proximal tubular epithelial cells of rats（Rattus norvegicus）. Histopathological examination illustrated that OTA induced cytoplasmic vacuolization and karyomegaly in proximal tubular epithelial cells. 1H NMR showed OTA could lead to the changes in metabolism of rats for the content of lactate, threonine, liquid, high-density lipoprotein（HDL） in plasma varied, which illustrated that OTA influenced some metabolic pathways of glycometabolism and lipid metabolism. This finding can provide valuable information to understand the metabolism of OTA from the perspective of metabolotics.

关 键 词：赭曲霉毒素A 血浆 代谢组学 核磁共振扫描(NMR) 

分 类 号：Q493[生物学—生理学]