响应面法优化BacillusamyloliquefaciensZJHD-06产类细菌素发酵培养基  

Optimization of fermentation medium of Bacillus amyloliquefaciens ZJHD-06 by response surface methodology

作　　者：安俊莹[1] 刘颖[1] 朱雯娟[1] 胡雪琼[1] 叶莉珍[1] 

机构地区：[1]广东省水产品加工与安全重点实验室,广东省普通高等学校水产品深加工重点实验室,广东海洋大学食品科技学院,广东湛江524000

出　　处：《食品工业科技》2014年第1期191-195,共5页Science and Technology of Food Industry

基　　金：广东省自然科学基金(S2011010000328);广东省科学技术厅(粤科规划字[2009]198号)

摘　　要：以分离自海洋宝石石斑鱼肠道的一种解淀粉芽孢杆菌ZJHD-06所产类细菌素为研究对象,以单核增生李斯特菌为抗菌活性测试指示菌,以抑菌圈直径大小为指标,采用响应面法对菌株ZJHD-06产类细菌素的发酵培养基成分进行优化。通过Plackett-Burman实验筛选出对类细菌素产量具有显著影响的因素,再通过最陡爬坡实验确定显著因素的中心水平,最后进行Box-Behnken实验设计,最终得到的最佳配比为葡萄糖浓度4.80%,蛋白胨浓度0.47%,酵母浸膏浓度0.44%,NaCl、K2HPO4和MgSO4·7H2O浓度分别为1.0%、0.01%、0.03%。在该培养基配比下,发酵上清液的抑菌圈直径增加了27.8%。The fermentation medium of Bacillus amyloliquefacien ZJHD-06 isolated from intestine of Epinephelus areolatus was optimized for a bacteriocin-fike substance（BLS）production.Bacteriostatic circle diameter was used as evaluation index against L.monocytogenes ATCC 35152.Firstly,the notable factors were screened by Plackett- Burman experiment design.Then,the path of steepest ascent was undertaken to approach the optimal region of the significant factors.Finally,Box-Behnken design was adopted to identify optimal proportion.The results showed the optimal proportions were glucose 4.80%, peptone 0.47%, yeast extract 0.44%, NaCl 1.0%, K2HPO＂ 0.01% and MgSQ-TH20 0.03%.The bacteriostatic circle diameter of the fermentation supernatant was increased by 2？.8% using the optimal proportions.

关 键 词：响应面 类细菌素 发酵培养基 

分 类 号：TS201.1[轻工技术与工程—食品科学]